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H2AX antibody | antibody review based on formal publications
H2AFX antibodies
This is a review about H2AX antibodies, based on 11 published articles using H2AX antibodies in western blot, immunohistochemistry, immunoprecipitation, immunocytochemistry, and other immunological technologies. It is aimed to help ExactAntigen visitors find the most suited H2AX antibody. Information in this review (with links to publications) can be searched freely.
ChIP assay (chip), immunocytochemistry (ic), immunoprecipitation (ip), western blot (wb)
MILLIPORE    search Millipore H2AFX products
includes other brands or spellings
chip, ic, ip    Upstate Biotechnology rabbit anti-gamma-H2AX and mouse anti-gamma-H2AX (clone S139) antibodies were used in immunoprecipitation, immunocytochemistry and ChIP assay to study the replicative senescence triggered by DNA damage checkpoint kinase Chk2.
ic, wb    Upstate cell signaling solutions antibody against gamma-H2AX was used in western blot and immunocytochemistry to detect gamma-H2AX in human fibroblast cell line DR95.
ic    Cell Signaling anti-gamma-H2AX antibody was used in immunocytochemistry to study the regulation of human Rif1, ortholog of a yeast telomeric protein by ATM and 53BP1 and functions in the S-phase checkpoint.
ic    Upstate Cell Signaling Solutions anti-gammaH2AX was used in immunocytochemistry to stain for gammaH2AX localization in HeLa cells.
ic    Upstate Cell Signaling Solutions anti-phospho-H2AX monoclonal antibodies were used in immunocytochemistry to detect the localization of phosphorylated (gamma) H2AX in human HeLa cells, MEF (mouse embryonic fibroblast) cells and mouse NIH3T3 cells.
ic    Upstate anti-phosphohistone H2A.X antibody (S139) antibody was used in immunocytochemistry to identify ATM domains for nuclear localization and chromatin association.
wb    Upstate Biotechnology anti-phospho-H2AX antibody (catalog no. 07-164) was used in western blot to study the function of Frag1 protein in higher eukaryotes.
wb    Upstate H2AX-S139 antibody was used in western blot to study the effect of Epstein-Barr virus lytic replication on ATM checkpoint signal transduction.
wb    Upstate Biotechnology antibody against phospho-H2AX was used in western blot to detect H2AX phosphorylation in human HCT116 and 293T cells.
wb    Upstate Biotechnology mouse monoclonal anti-phospho-H2AX (catalog number 05-636) antibody was used in western blot to study the benzo[a]pyrene-dihydrodiol epoxide (BPDE) -induced S-phase checkpoint.
TREVIGEN    search Trevigen H2AFX products
wb    Trevigen gamma-H2AX antibody was used in western blot to study p53 mitochondria translocation.


Articles Reviewed
1. Jacob G Robison et al. Replication protein A and the Mre11.Rad50.Nbs1 complex co-localize and interact at sites of stalled replication forks. 2004
2. Véronique Gire et al. DNA damage checkpoint kinase Chk2 triggers replicative senescence. 2004
3. Joshua Silverman et al. Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. 2004
4. Ayumi Kudoh et al. Epstein-Barr virus lytic replication elicits ATM checkpoint signal transduction while providing an S-phase-like cellular environment. 2005
5. Yoshimi Arima et al. Transcriptional blockade induces p53-dependent apoptosis associated with translocation of p53 to mitochondria. 2005
6. Xiaohui Bi et al. DNA polymerase kappa is specifically required for recovery from the benzo[a]pyrene-dihydrodiol epoxide (BPDE)-induced S-phase checkpoint. 2005
7. Fiona Pryde et al. 53BP1 exchanges slowly at the sites of DNA damage and appears to require RNA for its association with chromatin. 2005
8. David B Young et al. Identification of domains of ataxia-telangiectasia mutated required for nuclear localization and chromatin association. 2005
9. Hideshi Ishii et al. Frag1, a homolog of alternative replication factor C subunits, links replication stress surveillance with apoptosis. 2005
10. Hui Zhong et al. Rad50 depletion impacts upon ATR-dependent DNA damage responses. 2005
11. Amélie Rodrigue et al. Interplay between human DNA repair proteins at a unique double-strand break in vivo. 2006


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