| cat_no: | | T8397 |
| name: | | Thrombin from murine plasma |
| gene id: | | mouse F2(14061) |
| link: | | www.sigmaaldrich.com/catalog/search ... |
| analysis note: | | The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations. |
| application: | | Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. |
| biochem/physiol actions: | | Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B. |
| brand: | | SIAL |
| eg_ec_no: | | 232-648-7 |
| form: | | lyophilized powder |
| mdl_no: | | MFCD02179677 |
| name_suffix: | | lyophilized powder, ~1,000 NIH units/mg protein (biuret) |
| other notes: | | View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer. |
| physical form: | | Lyophilized from saline sodium citrate buffer, pH 6.5 |
| prod_type: | | Chemical |
| rtecs_no: | | XO8950000 |
| s_codes: | | 22-24/25 |
| storage: | | -20C |
| storage temp: | | -20C |
| syns: | | Factor IIa |
| wgk: | | 2 |
| more information: | | company product webpage |