| name: | Monoclonal Anti-Acetylated Tubulin antibody produced in mouse |
| cat_no: | T7451 |
| gene id: | human MAPT(4137);mouse Mapt(17762);rat Mapt(29477) |
| brand: | SIGMA |
| link: | www.sigmaaldrich.com/catalog/search ... |
| prod_type: | Chemical |
| name_suffix: | ~1 mg/mL, clone 6-11B-1, purified immunoglobulin, buffered aqueous solution |
| storage temp.: | -20C |
| storage: | -20C |
| form: | buffered aqueous solution |
| unspsc_code: | 12352203 |
| physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| shipped in: | dry ice |
| antibody form: | purified immunoglobulin |
| application(s): | radioimmunoassay: suitable;immunohistochemistry: suitable;electron microscopy: suitable;immunoblotting: 0.03-0.06 mug/mL using total rat brain extract;dot blot: suitable |
| species reactivity: | human, mouse, pig, bovine, rat, hamster, monkey, chicken, frog, invertebrates, plant, protista |
| immunogen: | acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). |
| wgk: | 3 |
| specificity: | The antibody has been used to detect acetylated alpha-tubulins from many organisms including protista, plants, invertebrates, and vertebrates. The antibody recognizes an epitope located on the alpha3 isoform of Chlamydomonas axonemal alpha-tubulin, within four residues of Lys40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of alpha-tubulins, but the corresponding region is markedly divergent in some alpha-tubulin isoforms from chicken, Drosophila, and yeast. Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK2. |
| clone: | 6-11B-1 |
| concentration: | ~1 mg/mL |
| isotype: | IgG2b |
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more information: | company product webpage |
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