| cat_no: | | T0157 |
| name: | | Tumor Necrosis Factor-alpha human |
| gene id: | | human TNF(7124) |
| link: | | www.sigmaaldrich.com/catalog/search ... |
| assay: | | >=95% (SDS-PAGE) |
| biochem/physiol actions: | | Tumor necrosis factor-alpha, also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-alpha plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-alpha is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-alpha is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-alpha is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-alpha also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-alpha is used in clinical trials as an antitumor agent, Sigma's cytokine, growth factor and hormone products are for research only. TNF-alpha and the related molecule TNF-beta (LT-alpha) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-alpha share 79% amino acid sequence identity. Unlike human TNF-alpha, the mouse form is glycosylated. |
| brand: | | SIGMA |
| form: | | buffered aqueous solution |
| mdl_no: | | MFCD00148692 |
| mol wt: | | mol wt ~17.4 kDa |
| name_suffix: | | recombinant, expressed in yeast, buffered aqueous solution, cell culture tested |
| physical form: | | Solution in phosphate buffered saline containing 1 mg/ml bovine serum albumin. |
| prod_type: | | Chemical |
| purity: | | >=95% (SDS-PAGE) |
| recombinant: | | expressed in yeast |
| rtecs_no: | | MT6435000 |
| shipped in: | | dry ice |
| storage: | | -20C |
| storage temp: | | -20C |
| syns: | | TNF-alpha |
| total impurities: | | Endotoxin, tested |
| unspsc_code: | | 12352207 |
| wgk: | | 3 |
| more information: | | company product webpage |
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