| cat_no: | P8825 |
| name: | Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse |
| gene id: | human PCNA(5111) |
| link: | www.sigmaaldrich.com/catalog/search ... |
| antibody form: | ascites fluid |
| application: | immunoblotting: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using human tonsil sections; immunoprecipitation: suitable; microarray: suitable; indirect ELISA: suitable; immunohistochemistry (frozen sections): suitable; immunocytochemistry: suitable; flow cytometry: suitable |
| brand: | SIGMA |
| clone: | PC 10 |
| immunogen: | PCNA-Protein A fusion protein |
| isotype: | IgG2a |
| name_suffix: | clone PC 10, ascites fluid |
| prod_type: | Chemical |
| shipped in: | dry ice |
| species reactivity: | human, monkey, insect, yeast |
| specificity: | Recognizes the acidic, non-histone, auxiliary protein of DNA polymerase, PCNA, also known as polymerase delta accessory protein. Fixation duration can markedly affect the intensity of PCNA immunoreactivity. However, delay in fixation does not affect the immunoreactivity. Enzymatic treatment destroys staining. In immunocytochemical labeling of acetone-methanol, or methanol-fixed cells, the antibody shows granular staining throughout the nucleus (nucleolus and nucleoplasm). Specific staining is observed in proliferating cell nuclei, particularly in germinal centers, of a wide range of normal and malignant tissues. |
| storage: | -20C |
| storage temp: | -20C |
| syns: | Monoclonal Anti-Proliferating Cell Nuclear Antigen; Anti-PCNA |
| unspsc_code: | 12352203 |
| wgk: | 3 |
|