| request information: |
|
| name: | PKD2 Active human |
| cat_no: | P7623 |
| gene id: | human PRKD2(25865) |
| brand: | SIGMA |
| prod_type: | Chemical |
| name_suffix: | recombinant, expressed in Escherichia coli, >=70% (SDS-PAGE), aqueous glycerol solution |
| storage temp.: | -70C |
| storage: | -70C |
| form: | aqueous glycerol solution |
| physical form: | Solution of 50 mul in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25Â mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol. |
| shipped in: | dry ice |
| syns: | Protein Kinase D2 |
| unspsc_code: | 12352209 |
| wgk: | 1 |
| mdl_no: | MFCD07371065 |
| application: | Kinase activity is measured as the molar amount of phosphate incorporated into the CREBTIDE substrate peptide (KRREILSRRPSYR), per minute per mg protein at 30 C using a final concentration of 50 ÂuM [32P] ATP. |
| mol wt: | protein apparent mol wt ~140 kDa |
| biochem/physiol actions: | PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation. |
| purity: | >=70% (SDS-PAGE) |
| assay: | >=70% (SDS-PAGE) |
order or
more information: | company product webpage |
|