| cat_no: | P247 |
| name: | Monoclonal Anti-phospho-CaM Kinase IIalpha (pThr286) antibody produced in mouse |
| gene id: | rat Camk2a(25400); mouse Camk2a(12322) |
| link: | www.sigmaaldrich.com/catalog/search ... |
| antibody form: | purified immunoglobulin |
| application: | immunoblotting: 1:2,000; indirect immunofluorescence: 1:100 using rat hippocampus (cold methanol fixation) |
| brand: | SIGMA |
| clone: | 22B1 |
| form: | buffered aqueous solution |
| immunogen: | phosphorylated (at Thr286) synthetic phosphopeptide corresponding to amino acids of rat and mouse CaM kinase IIalpha subunit that contains threonine 286. |
| isotype: | IgG1 |
| mdl_no: | MFCD01322970 |
| mol wt: | antigen mol wt ~50 kDa |
| name_suffix: | clone 22B1, purified immunoglobulin, buffered aqueous solution |
| physical form: | Solution in phosphate buffered saline containing 0.05% sodium azide. |
| prod_type: | Chemical |
| shipped in: | dry ice |
| species reactivity: | rat, mouse |
| specificity: | The antibody reacts specifically with both the recombinant and native rat phosphorylated CaM (Ca2+/calmodulin) dependent kinase IIalpha subunit. It does not cross-react with the non-phosphorylated alpha subunit or with the beta subunit (60 kDa) in either phosphorylation state. By immunofluorescence on rat hippocampal cells, the antibody labels cell somas and neuropil areas, while nuclei are only lightly stained. |
| storage: | -20C |
| storage temp: | -20C |
| unspsc_code: | 12352203 |
| wgk: | - |