| request information: |
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| name: | Anti-Nucleolin antibody produced in rabbit |
| cat_no: | N2662 |
| gene id: | human NCL(4691); mouse Ncl(17975); rat Ncl(25135) |
| brand: | SIGMA |
| prod_type: | Chemical |
| name_suffix: | affinity isolated antibody, buffered aqueous solution |
| storage temp.: | -20C |
| storage: | -20C |
| form: | buffered aqueous solution |
| physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| antibody form: | affinity isolated antibody |
| shipped in: | dry ice |
| species reactivity: | rat, mouse, human |
| application(s): | indirect immunofluorescence: 10-20 mug/mL using human HeLa cells; immunoblotting: 0.5-1.0 mug/mL using whole extract of mouse NIH3T3 cells or rat PC12 cells; immunoprecipitation: 1-2 mug using 250 mug RIPA lysate of human HeLa cells |
| immunogen: | synthetic peptide corresponding to amino acid residues 2-17 of human nucleolin with C-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by 4 amino acids in rat and mouse nucleolin and by 1 amino acid in the rat homolog protein NRP (nucleolin related protein). |
| syns: | Anti-C23 |
| unspsc_code: | 12352203 |
| mdl_no: | MFCD02686475 |
| mol wt: | antigen mol wt 105 kDa |
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more information: | company product webpage |