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| name: | Monoclonal Anti-Nitric Oxide Synthase, Universal antibody produced in mouse |
| cat_no: | N218 |
| gene id: | rat Nos1(24598); mouse Nos1(18125) |
| brand: | SIGMA |
| prod_type: | Chemical |
| name_suffix: | clone NOS-3F7-B11-B5, ascites fluid |
| storage temp.: | -20C |
| storage: | -20C |
| physical form: | Solution in phosphate buffered saline, containing 0.05% sodium azide. |
| antibody form: | ascites fluid |
| shipped in: | dry ice |
| species reactivity: | rat, mouse, bovine |
| application(s): | immunohistochemistry (frozen sections): 1:100; immunoblotting: 1:250 |
| immunogen: | bovine cerebellum NOS. |
| unspsc_code: | 12352203 |
| mdl_no: | MFCD01324876 |
| clone: | NOS-3F7-B11-B5 |
| isotype: | IgM |
| specificity: | The antibody reacts with iNOS, eNOS and nNOS from rat and bovine, and with iNOS in induced RAW 264.7 mouse macrophages. In immunoblotting, the antibody detects a band at 130 kDa representing iNOS in samples first induced with IFN (interferon) and LPS (lipopolysaccharides). It also detects a band at 155 kDa in tissues expressing nNOS and a band at 140 kDa in tissues expressing eNOS. The antibody may be used to localize and detect the three isoforms of nitric oxide synthase (NOS): neuronal or brain (nNOS or bNOS), endothelial (eNOS) and inducible (iNOS). |
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more information: | company product webpage |
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