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| name: | Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) antibody produced in mouse |
| cat_no: | M9692 |
| gene id: | human MAPK3(5595); rat Mapk3(50689); rat Mapk1(116590); mouse Mapk1(26413); mouse Mapk3(26417); human MAPK1(5594) |
| brand: | SIGMA |
| prod_type: | Chemical |
| name_suffix: | 1.5-2 mg/mL, clone MAPK-YT, purified immunoglobulin, buffered aqueous solution |
| storage temp.: | -20C |
| storage: | -20C |
| form: | buffered aqueous solution |
| physical form: | Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| antibody form: | purified immunoglobulin |
| shipped in: | dry ice |
| species reactivity: | human, rat, mouse, Drosophila, yeast, bovine, hamster, Xenopus, Caenorhabditis elegans |
| application(s): | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation: suitable; indirect ELISA: suitable; microarray: suitable; immunoblotting: 0.5-1 mug/mL using a whole cell extract of RAT-1 cells treated with vanadate and H2O2.; immunocytochemistry: suitable |
| immunogen: | synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of the ERK-activation loop. |
| syns: | Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2); Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) |
| unspsc_code: | 12352203 |
| clone: | MAPK-YT |
| isotype: | IgG1 |
| mol wt: | antigen ERK-2 mol wt 42 kDa; antigen ERK-1 mol wt 44 kDa |
| specificity: | The antibody reacts specifically with the diphosphorylated form of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated or the monophosphorylated forms of MAP kinase or the diphosphorylated forms of JNK and p38 MAP kinase. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase. |
| concentration: | 1.5-2 mg/mL |
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more information: | company product webpage |