| cat_no: | | M9069 |
| name: | | MRP2 human |
| gene id: | | human ABCC2(1244) |
| link: | | www.sigmaaldrich.com/catalog/search ... |
| analysis note: | | The quantity of transported molecules can be determined by methods such as HPLC, LC/MS/MS separation and detection, and also by labeling with fluorescent or radioactive (3H-beta-estradiol 17-(beta-D-glucuronide) tags. MRP2 mediates the transport of beta-estradiol 17-(beta-D-glucuronide) (E217betaG) very efficiently. Compounds that interact with the transporter modulate the initial rate of E217betaG transport measured without any other compounds added. If a substance is a transported substrate of the transporter, it might compete with E217betaG, thus reducing the rate of E217betaG transport. If a compound is an inhibitor of the transporter, it will block the transport of E217betaG into the membrane vesicles. Some compounds can be co-transported with E217betaG increasing the rate of E217betaG transport compared to the control level. |
| biochem/physiol actions: | | The vesicular transport assay determines the interaction of compounds with the MRP2 transporter. The interaction is detected by changes in the initial rate of 3H-beta-estradiol 17-(beta-D-glucuronide) transport by MRP2 into membrane vesicles purified from Sf9 cells expressing the transporters. Membrane preparations from infected cells always contain some closed membrane vesicles that have an inside-out orientation (5-10% of total lipid). In the case of these inside-out vesicles, transport of substrates across the membrane takes molecules from the surrounding buffer and transports them into the vesicles. |
| brand: | | SIGMA |
| form: | | membrane preparation |
| legal information: | | Distributed for SOLVO Biotechnology, Inc. |
| name_suffix: | | membrane preparation, for Vesicular Transport, recombinant, expressed in Sf9 cells |
| physical form: | | Supplied as isolated Sf9 cell membranes containing human MRP2 suspended in TMEP solution. |
| prod_type: | | Chemical |
| recombinant: | | expressed in Sf9 cells |
| shipped in: | | dry ice |
| storage: | | -70C |
| storage temp: | | -70C |
| unspsc_code: | | 12352202 |
| wgk: | | 1 |
| more information: | | company product webpage |