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| name: | Monoclonal Anti-MAP Kinase, Non-Phosphorylated ERK antibody produced in mouse |
| cat_no: | M3807 |
| gene id: | human MAPK3(5595); rat Mapk3(50689); rat Mapk1(116590); human MAPK1(5594) |
| brand: | SIGMA |
| prod_type: | Chemical |
| name_suffix: | ~2 mg/mL, clone ERK-NP2, purified immunoglobulin, buffered aqueous solution |
| storage temp.: | -20C |
| storage: | -20C |
| form: | buffered aqueous solution |
| physical form: | Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| antibody form: | purified immunoglobulin |
| shipped in: | dry ice |
| species reactivity: | human, rat |
| application(s): | indirect ELISA: suitable; microarray: suitable; immunocytochemistry: suitable; immunoblotting: 5-25 mug/mL using rat brain extract |
| immunogen: | synthetic peptide HTGFLTEYVAT, corresponding to the non-phosphorylated form of ERK-activation loop. |
| unspsc_code: | 12352203 |
| wgk: | 3 |
| mdl_no: | MFCD02096849 |
| clone: | ERK-NP2 |
| isotype: | IgG1 |
| mol wt: | antigen, ERK-1 mol wt 44 kDa; antigen, ERK-2 mol wt 42 kDa |
| specificity: | The antibody reacts specifically with the non-phosphorylated, non-activated form of MAP kinase (ERK-1 and ERK-2). Weak cross-reaction is observed with monophosphorylated (threonine or tyrosine), but not with diphosphorylated peptides of MAPK. The antibody does not recognize JNK or p38 MAPK. The epitope recognized by the antibody contains non-phosphorylated threonine 183 and tyrosine 185 which resides within the ERK-activation loop (e.g., amino acids 178-188 in ERK-2). |
| concentration: | ~2 mg/mL |
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more information: | company product webpage |