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| name: | Monoclonal Anti-MAP Kinase, Monophosphorylated Tyrosine antibody produced in mouse |
| cat_no: | M3682 |
| gene id: | human MAPK3(5595); rat Mapk3(50689); rat Mapk1(116590); human MAPK1(5594) |
| brand: | SIGMA |
| prod_type: | Chemical |
| name_suffix: | ~2 mg/mL, clone ERK-PY193, purified immunoglobulin, buffered aqueous solution |
| storage temp.: | -20C |
| storage: | -20C |
| form: | buffered aqueous solution |
| physical form: | Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| antibody form: | purified immunoglobulin |
| shipped in: | dry ice |
| species reactivity: | human, rat |
| application(s): | microarray: suitable; immunocytochemistry: suitable; capture ELISA: suitable; immunoblotting: 1-5 mug/mL using cell extract of rat fibroblasts cell line, Rat1, activated with sorbitol |
| immunogen: | synthetic peptide HTGFLTEpYVAT, corresponding to the phosphorylated form of ERK-activation loop. |
| syns: | Anti-pY-ERK |
| unspsc_code: | 12352203 |
| wgk: | 3 |
| mdl_no: | MFCD02096848 |
| clone: | ERK-PY193 |
| isotype: | IgG1 |
| mol wt: | antigen, ERK-1 mol wt 44 kDa; antigen, ERK-2 mol wt 42 kDa |
| specificity: | The antibody reacts with the monophosphorylated tyrosine form of MAP kinases (ERK1 and ERK2). It does not recognize the non-phosphorylated, diphosphorylated, and the monophosphorylated threonine forms of ERK/MAP kinases, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated tyrosine residue within the regulatory site of MAP kinase (e.g.,Tyr185 in ERK-2). Cross-reactivity has been observed with the monophosphorylated tyrosine peptide of JNK. |
| concentration: | ~2 mg/mL |
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more information: | company product webpage |