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| name: | Monoclonal Anti-MAP Kinase, Monophosphorylated Threonine antibody produced in mouse |
| cat_no: | M3557 |
| gene id: | human MAPK3(5595); rat Mapk3(50689); rat Mapk1(116590); human MAPK1(5594); mouse Mapk1(26413); mouse Mapk3(26417) |
| brand: | SIGMA |
| prod_type: | Chemical |
| name_suffix: | ~2 mg/mL, clone ERK-YNP, purified immunoglobulin, buffered aqueous solution |
| storage temp.: | -20C |
| storage: | -20C |
| form: | buffered aqueous solution |
| physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| antibody form: | purified immunoglobulin |
| shipped in: | dry ice |
| species reactivity: | human, rat, mouse |
| application(s): | immunoblotting: 5-20 mug/mL using rat brain extract; microarray: suitable; immunocytochemistry: suitable; capture ELISA: suitable |
| immunogen: | synthetic peptide HTGFLTEYVAT, corresponding to the non-phosphorylated form of ERK-activation loop. |
| syns: | Anti-pT-ERK |
| unspsc_code: | 12352203 |
| wgk: | 3 |
| mdl_no: | MFCD01633612 |
| clone: | ERK-YNP |
| isotype: | IgG1 |
| mol wt: | antigen, ERK-1 mol wt 44 kDa; antigen, ERK-2 mol wt 42 kDa |
| specificity: | The antibody reacts with the monophosphorylated threonine MAP kinase (ERK-1 and ERK-2) proteins and weaker with the non-phosphorylated forms of MAP kinases. It does not recognize the diphosphorylated, and the monophosphorylated tyrosine forms of the ERK/MAPK proteins, or the JNK and p38 MAPK. The epitope recognized by the antibody contains the threonine residue within the regulatory site of MAP kinase (e.g.,Thr183 in ERK-2). |
| concentration: | ~2 mg/mL |
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more information: | company product webpage |