| cat_no: | | H5529 |
| name: | | Heregulin-alpha, EGF Domain human |
| gene id: | | human NRG1(3084) |
| link: | | www.sigmaaldrich.com/catalog/search ... |
| analysis note: | | The bioactivity is tested in culture using a proliferation assay with the human cell line MCF-7. |
| assay: | | >=97% (SDS-PAGE) |
| biochem/physiol actions: | | Heregulin (HRG) is the human homologue to the neu differentiation factor (NDF) in rat. The heregulin family members contain one EGF-like motif and an IgD-like motif in the extracellular domain. They bind to ErbB-2, ErbB-3, and ErbB-4 (receptors closely related to EGFR). HRG-alpha and HRG-beta isoforms differ slightly in the EGF domain due to alternate splicing. HRG-beta isoforms are further subdivided into beta1, beta2, and beta3 isoforms, which show identical binding and activation characteristics. Both alpha and beta HRG isoforms bind to ErbB-3 and ErbB-4 homodimers, but not directly to ErbB-2. HRG-alpha binding to ErbB-3 and ErbB-4 is reported to be approximately 100-fold weaker than that of HRG-beta. When ErbB-2 is combined into a heterodimer with ErbB-3 or ErbB-4, the binding affinities of both alpha and beta isoforms are substantially improved. HRGs are mitogenic for Schwann cells in culture and weakly to moderately mitogenic for a variety of epithelial cells, including mammary, ovarian, lung, and gastric cells. HRGs inhibit proliferation and induce differentation in some tumor cell lines, such as certain mammary tumor cells, which are arrested at the G2M phase. HRGs also induce expression of acetylcholine receptors and possibly other molecules in muscle cells at newly formed neuromuscular synapses, suggesting they may play a role in neuromuscular synapse maturation and maintenance. |
| brand: | | SIGMA |
| form: | | lyophilized powder |
| mdl_no: | | MFCD00677127 |
| mol wt: | | protein mol wt ~7 kDa |
| name_suffix: | | >=97% (SDS-PAGE), recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested |
| physical form: | | Lyophilized from a 0.2 mum filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin. |
| prod_type: | | Chemical |
| purity: | | >=97% (SDS-PAGE) |
| recombinant: | | expressed in Escherichia coli |
| storage: | | -20C |
| storage temp: | | -20C |
| syns: | | HRG-alpha |
| total impurities: | | Endotoxin, tested |
| unspsc_code: | | 12352207 |
| wgk: | | 3 |
| more information: | | company product webpage |
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