| cat_no: | H0273 |
| name: | Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse |
| gene id: | rat Hspb1(24471); mouse Hspb1(15507); human HSPB1(3315) |
| link: | www.sigmaaldrich.com/catalog/search ... |
| antibody form: | ascites fluid |
| application: | immunoblotting: 1:500 using turkey gizzard extract; indirect ELISA: suitable; radioimmunoassay: suitable; indirect immunofluorescence: suitable using formalin-fixed, heat-shocked cultured chick embryo fibroblasts; immunohistochemistry: suitable using frozen sections of chicken gizzard smooth muscle |
| brand: | SIGMA |
| clone: | IAP-28 |
| immunogen: | partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle. |
| isotype: | IgG1 |
| mdl_no: | MFCD02189139 |
| mol wt: | antigen mol wt 25 kDa |
| name_suffix: | clone IAP-28, ascites fluid |
| prod_type: | Chemical |
| shipped in: | dry ice |
| species reactivity: | human, bovine, rabbit, guinea pig, chicken, turkey, rat, mouse, frog |
| specificity: | The antibody also reacts with a dimer of the HSP 25 (approx. 58 kDa) appearing in preparations stored for prolonged periods prior to electrophoretic analysis. A major proteolytic fragment of 22 kDa may also be detected. The antibody suppresses the inhibition of actin elongation induced by the 25 kDa IAP. The antibody displays a punctate or fine fibrillary pattern of labeling throughout the cytoplasm in heat-shocked cells and co-localization with mitochondria. Reactivity has been observed with HSP 25 of various organs. |
| storage: | -20C |
| storage temp: | -20C |
| unspsc_code: | 12352203 |
| wgk: | 3 |