| cat_no: | F0167 |
| name: | Monoclonal Anti-Proliferating Cell Nuclear Antigen-FITC antibody produced in mouse |
| gene id: | human PCNA(5111) |
| link: | www.sigmaaldrich.com/catalog/search ... |
| antibody form: | purified immunoglobulin |
| application: | flow cytometry: suitable; This product may be used in flow cytometry when applying special fixation procedures (see Landberg, G., and Roos, G., and Beppu, T., et al.) and in double labeling experiments. |
| brand: | SIGMA |
| clone: | PC 10 |
| form: | buffered aqueous solution |
| immunogen: | PCNA-Protein A fusion protein |
| isotype: | IgG2a |
| name_suffix: | clone PC 10, purified immunoglobulin, buffered aqueous solution |
| physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| prod_type: | Chemical |
| shipped in: | wet ice |
| species reactivity: | human, monkey, insect, yeast |
| specificity: | Recognizes the acidic, non-histone, auxiliary protein of DNA polymerase, PCNA, also known as polymerase delta accessory protein. Fixation duration can markedly affect the intensity of PCNA immunoreactivity. However, delay in fixation does not affect the immunoreactivity. Enzymatic treatment destroys staining. In immunocytochemical labeling of acetone-methanol, or methanol-fixed cells, the antibody shows granular staining throughout the nucleus (nucleolus and nucleoplasm). Specific staining is observed in proliferating cell nuclei, particularly in germinal centers, of a wide range of normal and malignant tissues. |
| storage: | 2-8C |
| storage temp: | 2-8C |
| syns: | Monoclonal Anti-Proliferating Cell Nuclear Antigen; Anti-PCNA-FITC |
| unspsc_code: | 12352203 |
| wgk: | 3 |
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