| cat_no: | | A8729 |
| name: | | Akt1 Active human |
| gene id: | | human AKT1(207) |
| link: | | www.sigmaaldrich.com/catalog/search ... |
| application: | | Can be used in kinase activity assays, which measure the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF) per minute per mg protein at 30 C using a final concentration of 50 µM [32P]-ATP. |
| assay: | | >=85% (SDS-PAGE) |
| biochem/physiol actions: | | Akt1 is an isoform of multifunctional Akt kinase, which upon activation becomes involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, cell motility and a number of metabolic effects of insulin. |
| brand: | | SIGMA |
| form: | | aqueous glycerol solution |
| mdl_no: | | MFCD02685868 |
| mol wt: | | protein apparent mol wt ~80 kDa |
| name_suffix: | | aqueous glycerol solution, recombinant, expressed in Escherichia coli, protein apparent mol wt ~80 kDa, >=85% (SDS-PAGE) |
| other notes: | | GST-tagged human recombinant full length Akt1 |
| physical form: | | Supplied as a solution at 15 mug in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA and 30% glycerol. |
| prod_type: | | Chemical |
| purity: | | >=85% (SDS-PAGE) |
| recombinant: | | expressed in Escherichia coli |
| shipped in: | | dry ice |
| storage: | | -70C |
| storage temp: | | -70C |
| syns: | | PKB alpha; Protein Kinase B-alpha |
| unspsc_code: | | 12352209 |
| wgk: | | 1 |
| more information: | | company product webpage |