| cat_no: | A5441 |
| name: | Monoclonal Anti-beta-Actin antibody produced in mouse |
| gene id: | human ACTB(60); rat Actb(81822); mouse Actb(11461) |
| link: | www.sigmaaldrich.com/catalog/search ... |
| antibody form: | ascites fluid |
| application: | indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts; indirect ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoblotting: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts |
| brand: | SIGMA |
| clone: | AC-15 |
| immunogen: | slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. |
| isotype: | IgG1 |
| mol wt: | antigen mol wt 42 kDa |
| name_suffix: | clone AC-15, ascites fluid |
| prod_type: | Chemical |
| shipped in: | dry ice |
| species reactivity: | human, bovine, sheep, pig, rabbit, feline, canine, mouse, rat, guinea pig, chicken, carp, Hirudo medicinalis, Drosophila |
| specificity: | The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin's solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.; In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. |
| storage: | -20C |
| storage temp: | -20C |
| syns: | Monoclonal Anti-beta-Actin |
| unspsc_code: | 12352203 |
| wgk: | 3 |
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