| name: | Monoclonal Anti-beta-Actin antibody produced in mouse |
| cat_no: | A5316 |
| gene id: | human ACTB(60);mouse Actb(11461);rat Actb(81822) |
| brand: | SIGMA |
| link: | www.sigmaaldrich.com/catalog/search ... |
| prod_type: | Chemical |
| name_suffix: | clone AC-74, ascites fluid |
| storage temp.: | -20C |
| storage: | -20C |
| unspsc_code: | 12352203 |
| shipped in: | dry ice |
| antibody form: | ascites fluid |
| application(s): | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable;indirect ELISA: suitable;immunoblotting: 1:5,000 using cultured human or chicken fibroblast cell extracts;indirect immunofluorescence: 1:1,000 using cultured human or chicken fibroblasts |
| species reactivity: | wide range, Drosophila, Hirudo medicinalis, bovine, carp, cat, chicken, canine, guinea pig, human, mouse, pig, rabbit, rat, sheep |
| immunogen: | slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. |
| mdl_no: | MFCD00164531 |
| wgk: | 3 |
| mol wt: | antigen mol wt 42 kDa |
| specificity: | Recognizes an epitope located on the N-terminal end of the beta-isoform of actin. It specifically labels beta-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with beta-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin's solutions may be also used as fixatives. The antibody can be used as probes for beta-actin as an internal control in immunoblotting. |
| clone: | AC-74 |
| isotype: | IgG2a |
| other notes: | To view an Actin antibody selection guide, please click here. |
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more information: | company product webpage |
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