| request information: | |
| name: | Anti-Actin (20-33) antibody produced in rabbit |
| cat_no: | A5060 |
| gene id: | mouse Acta1(11459); rat Acta1(29437); human ACTA1(58) |
| brand: | SIGMA |
| prod_type: | Chemical |
| name_suffix: | IgG fraction of antiserum, buffered aqueous solution |
| storage temp.: | -20C |
| storage: | -20C |
| form: | buffered aqueous solution |
| physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative. |
| antibody form: | IgG fraction of antiserum |
| shipped in: | dry ice |
| species reactivity: | invertebrates, wide range (animal) |
| application(s): | microarray: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human or animal tissue sections; immunohistochemistry (frozen sections): suitable using human or animal tissue sections; indirect immunofluorescence: 1:200 using cultured human fibroblasts; immunoblotting: 1:250 using human and animal tissue extracts |
| immunogen: | N-terminal actin peptide (amino acid residues 20-33) attached to a multiple antigen peptide (MAP) backbone. |
| unspsc_code: | 12352203 |
| mdl_no: | MFCD01092261 |
| mol wt: | antigen mol wt 42 kDa |
| specificity: | The antibody shows a broad reactivity among actin isoforms and across a range of organisms. It specifically stains typical stress fibers in cultured cells using an indirect immunofluorescent labeling technique and can be used as a probe of the N-terminal region of actin. |
order or more information: | company product webpage |