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| Catalog Code: | R-118-100 |
| Product Name: | Tyrosine Hydroxylase Antibody |
| Product Description: | Rabbit Polyclonal anti-Tyrosine Hydroxylase |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | A synthetic peptide (SPRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (61-77) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (30-46). |
| Specificity: | IHC on brain shows a pattern of staining specific for TH containing neurons. |
| Cross Reactivity: | This antibody is known to react with rat. Cross reactivity with other species has not yet been tested. |
| Packaging: | 0.1 ml protein A purified Rabbit antisera. |
| Uses: | IHC. A dilution of 1:2000 to 1: 5000 is recommended for this application. The optimal working dilution should be determined by the end user. This is a superb antibody for detection of tyrosine hydroxylase containing neurons exhibiting an intense labeling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals. Highly recommended. |
| Localization: | Accession Number: TY3H_HUMAN ; TY3H_RAT |
| Background: | Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality. |
| Purity: | protein A purified |
| Host Name: | Rabbit |
| Buffer: | Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. |
| Application Summary: | immunohistochemistry |
| Species Summary: | Rt, |
| Alternate Names: | anti-TH antibody, anti-Tyrosine hydroxylase antibody, anti-Tyrosine 3-monooxygenase antibody, anti-L-tyrosine hydroxylase antibody, anti-Tyrosine 3-hydroxylase antibody |
| Package Size: | 0.1 ml |
| Preservative: | No Preservative |
| Notes Main: | This product is distributed for biosensis Pty Ltd, Australia. For the most up-to-date information regarding this product, please go to biosensis' website: http://www.biosensis.com/product_info.php?products_id=195 |
| Product References: | 1. Mallett, J. Trends in Pharmacological Science. 17(4): 129-135, 1996. ; 2. Haavik, J. et al. Mol. Neurobiology 16(3) :285-309, 1998. ; 3. Lewis DA, et al, Neuroscience 54: 477-, 1993 ; 4. Kumer S.C. et al. Journal of Neurochemistry, 67(2) :443-462, 1996. ; 5. Haycock, J. Anal. Biochemistry 181: 259-266, 1989. ; 6. Haycock, J. Anal. Biochemistry 208: 397-399, 1993. ; 7. Renfroe, J.B., et al. Brain Res. Bull. 13: 109 - 126, 1984. ; 8. Xu, Z et a.l Neurosci. 82(3): 727, 1998 |
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more information: | company product webpage |