| CatalogCode: | NSB966 |
| ProductName: | PKC delta Antibody |
| Product Description: | Rabbit Polyclonal anti-PKC delta [Ser645] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic phosphopeptide derived from the region of human PKC delta that contains serine 645. |
| CrossReactivity: | Cross-reacts with Human. Rat and mouse (77% homologous) PKC delta have not been tested, but our peptide competition data (see Figure) suggest that they will not react strongly with this antibody. The peptide competition data (see Figure) suggest that this antibody may cross-react with PKC theta (62% homologous).Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: Use at a concentration of 0.35 - 1 ug/ml. Detects a band of approximately 77 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | U937 cells |
| Background: | Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. In contrast to other PKC isozymes where phosphorylation of the activation loop residue is essential for catalytic activity, phosphorylation of the activation loop threonine 507 of PKC delta appears to modulate, but is not required for, catalytic activity. This phosphorylation may also regulate PKC delta stability and degradation. Autophosphorylation at serine 645 (serine 643 in mouse and rat) is not required for, but may modulate, catalytic activity and receptor dephosphorylation. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-PKC d antibody, anti-PKC-d antibody, anti-PKCd antibody, anti-PRKC d antibody, anti-PRKC delta antibody, anti-PRKC-d antibody, anti-PRKCd antibody, anti-Protein Kinase C delta antibody |
| ProteinTarget: | PKC delta phospho S645 |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Brodie C & Blumberg PM Regulation of cell apoptosis by protein kinase c delta. Apoptosis 8:19-27 (2003). Newton AC Regulation of the ABC kinases by phosphorylation: protein kinase C as a paradigm. Biochem J 370:361-71 (2003). Kikkawa U et al. Protein Kinase Cdelta (PKCdelta): Activation Mechanisms and Functions. J Biochem (Tokyo) 132:831-9 (2002). Shirai Y & Saito N Activation mechanisms of protein kinase C: maturation, catalytic activation, and targeting. J Biochem (Tokyo) 132:663-8 (2002). Stempka L et al. Requirements of protein kinase cdelta for catalytic function. Role of glutamic acid 500 and autophosphorylation on serine 643. J Biol Chem 274:8886-92 (1999). Li W et al. Identification of serine 643 of protein kinase C-delta as an important autophosphorylation site for its enzymatic activity. J Biol Chem 272:24550-5 (1997). |
| Phosphorylated: | Ser645 |
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