| CatalogCode: | NSB964 |
| ProductName: | PKC beta 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-PKC beta 2 [Thr641] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic phosphopeptide derived from a region of human PKC beta 2 that contains threonine 641. |
| CrossReactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | Detects a band of approximately 80 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and membrane associated. |
| Control: | K562 cells treated with PMA, a phorbol ester. |
| Background: | Protein Kinase C beta 2 (PKC beta 2) is an 80 kDa member of the conventional group (cPKCs: sensitive to diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion. PKC beta 2 is phosphorylated on three sites, threonine 500 in the activation loop, threonine 641 in the turn loop and serine 660 in the hydrophobic loop. Threonine 641 mediates PKC beta 2 binding to Hsp70 which regulates its stability and phosphorylation. Phosphorylation of threonine 641 is also crucial for PKC beta 2 proper subcellular localization and catalytic function as well as for its autophosphorylation on serine 660. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-PKC beta antibody; anti-PKCB antibody; anti-PKCB2 antibody; anti-PRKCB antibody; anti-PRKCB2 antibody; anti-Protein kinase C beta 1 polypeptide antibody; anti-Protein kinase C beta 2 antibody |
| ProteinTarget: | PKC beta 2 phospho T641 |
| PackageSize: | 0.1 ml |
| GeneralRef: | <b>General / background references:</b>Gao T & Newton AC The turn motif is a phosphorylation switch that regulates the binding of Hsp70 to protein kinase C. <i>J Biol Chem</i> <b>277</b>:31585-92 (2002). <PUBMED:12080070>Edwards AS <i>et al.</i> Carboxyl-terminal phosphorylation regulates the function and subcellular localization of protein kinase C betaII. <i>J Biol Chem</i> <b>274</b>:6461-8 (1999). <PUBMED:10037738>Keranen LM <i>et al.</i> Protein kinase C is regulated in vivo by three functionally distinct phosphorylations. <i>Curr Biol</i> <b>5</b>:1394-1403 (1995). <PUBMED:8749392>Newton AC Protein kinase C: structure, function, and regulation. <i>J Biol Chem</i> <b>270</b>:28495-8 (1995). <PUBMED:7499357> 5. Zhang, J., et al. (1994) Phosphorylation of Thr642 is an erly event in the processing of mewly synthesized protein kinase C B1 and is essential for its activation. JBC. 269(30): 19578-19584. |
| Phosphorylated: | Thr641 |