| CatalogCode: | NSB942 |
| ProductName: | PAK 1 + 2 + 3 Antibody |
| Product Description: | Rabbit Polyclonal anti-PAK 1 + 2 + 3 [Thr423] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PAK that contains threonine 423 (for PAK1). The sequence is conserved across many species for all three main isoforms of PAK. |
| CrossReactivity: | Human PAK1, PAK2, and PAK3. Other species (100% homologous) have not been tested, but are expected to react. |
| Packaging: | 0.1 ml Rabbit antisera. |
| Uses: | This antibody is suitable for use in Western blotting. The recommended concentration for use in Western blotting is 1:1,000. *The optimal concentration should be determined for each specific application. |
| Control: | His-tagged wild-type and S139A/T421A double mutant PAK3. |
| Background: | p21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK1, PAK2, and PAK3 (also known as a-PAK, g-PAK, and b-PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase-mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Host_Name: | Rabbit |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-p21 Activated Kinase 1 + 2 + 3 antibody |
| ProteinTarget: | PAK 1/2/3 Phosphospecific |
| PackageSize: | 0.1 ml |
| GeneralRef: | Zang, M., et al. (2002) Interaction between active Pak1 and Raf-1 is necessary for phosphorylation and activation of Raf-1. J. Biol. Chem. 277(6):4395-4405. Chong, C., et al. (2001) The mechanism of PAK activation. Autophosphorylation events in both regulatory and kinase domains control activity. J. Biol. Chem. 276(20):17347-17353. del Pozo, M.A., et al. (2000) Adhesion to the extracellular matrix regulates the coupling of the small GTPase Rac to its effector PAK. EMBO J. 19(9):2008-2014. Schurmann, A., et al. (2000) p21-activated kinase 1 phosphorylates the death agonist Bad and protects cells from apoptosis. Mol. Cell Biol. 20(2):453-461. Zenke, F.T., et al. (1999) Identification of a central phosphorylation site in p21-activated kinase regulating autoinhibition and kinase activity. J. Biol. Chem. 274(46):32565-32573. Gatti, A., et al. (1999) Multisite autophosphorylation of p21-activated protein kinase gamma-PAK as a function of activation. J. Biol. Chem. 274(12):8022-8028. |
| Phosphorylated: | Thr423 |
|