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| Catalog Code: | NSB936 |
| Product Name: | MLK3 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-MLK3 Phosphospecific [Thr227/Ser281] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 0.75 mg/ml |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human MLK3 that contains threonine 277 and serine 281. The sequence is conserved in mouse. |
| Cross Reactivity: | Human MLK3. Mouse (100% homologous) MLK3 has not been tested. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | The antibody has been used for Western blotting. No other applications have been tested.Recommended starting dilutions:Western Blot - 1:1000 *The optimal antibody concentration should be determined empirically for each specific application. |
| Localization: | Centrosome. Location is cell cycle dependent. |
| Background: | Members of the mixed-lineage kinase (MLK) family (including MLK1, MLK2, MLK3, and dual leucine zipper kinase [DLK]) are serine/threonine protein kinases that are expressed in multiple cell types. MLK3 is activated by phosphorylation in response to stress stimuli (e.g., inflammatory responses, UV, chemical stress) that are coupled to the small GTPase, Cdc42/rac. MLK3 is a multifunctional kinase that plays an essential role in several signaling pathways, including mitogen-activated protein kinase (i.e., activation of JNK and p38), IkappaB/NFkappaB, and p70 S6 kinase. Indeed MLK3 signaling occurs through multiple signaling domains in this protein kinase including (from N- to C-terminal) a glycine-rich domain, Src homology 3 (SH3) domain, a kinase domain, a zipper domain, a Cdc42/rac interactive binding (CRIB) domain and a Pro/Ser/Thr-rich domain. Phosphorylation of MLK3 occurs on multiple residues including threonine 277 and serine 281 within the activation loop of the kinase domain. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Host Name: | Rabbit |
| Buffer: | Dulbecco's phosphate buffered saline, with 1.0 mg/mL BSA (IgG, protease free) as a carrier |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Thr227/Ser281 |
| General References: | Vacratsis, P.O., et al. (2002) Identification of in vivo phosphorylation sites of MLK3 by mass spectrometry and phosphopeptide mapping. Biochemistry 41(17):5613-5624. Xu, Z., et al. (2001) The MLK family mediates c-Jun N-terminal kinase activation in neuronal apoptosis. Mol. Cell Biol. 21(14):4713-4724. Leung, I.W., and N. Lassam (2001) The kinase activation loop is the key to mixed lineage kinase-3 activation via both autophosphorylation and hematopoietic progenitor kinase 1 phosphorylation. J. Biol. Chem. 276(3):1961-1967. Hehner, S.P., et al. (2000) Mixed-lineage kinase 3 delivers CD3/CD28-derived signals into the IkappaB kinase complex. Mol. Cell Biol. 20(7):2556-2568. Tibbles, L.A., et al. (1996) MLK-3 activates the SAPK/JNK and p38/RK pathways via SEK1 and MKK3/6. EMBO J. 15(24):7026-7035. |
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