| CatalogCode: | NSB926 |
| ProductName: | RSK1 p90 Antibody |
| Product Description: | Rabbit Polyclonal anti-RSK1 p90 [Ser363] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (Human). |
| CrossReactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (91% identity with immunogen) and Rat (91% identity with immunogen) due to sequence homology. Expected to cross react with RSK2 (82% homologous) based on peptide competition and with RSK3 and RSK4 (both 73% homologous), though not tested.Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: Use at a dilution of 1/1000. Predicted molecular weight: 90 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Control: | A431 cells treated with 100 ng/mL PMA, a phorbol ester activator of protein kinase C, for 30 minutes |
| Background: | RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2 (MAPKAP-K1b), RSK3 (MAPKAP-K1c), and RSK4. RSK proteins are substrates for and downstream transducers of MAPK signaling proteins, primarily ERK1&2. RSK proteins possess two separate kinase domains, the C-terminal kinase domain and the N-terminal kinase domain, separated by a linker region containing a hydrophobic motif. Activation of RSK1 is a multi-step process involving phosphorylation of multiple residues within the three domains, changes in RSK protein localization, and modulation of RSK complex formation with ERK1&2 and/or 14-3-3 beta proteins. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation. ERK 1&2 phosphorylate threonine 573 of the C-terminal kinase domain, thereby stimulating this kinase activity and leading to phosphorylation of serines 363 and 380 within the linker region. This phosphorylation is required for activation of the N-terminal kinase domain by PDK1. The activated N-terminal kinase domain phosphorylates various substrates that include transporters, transcription factors, and transcription co-activators. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: 50% Glycerol, 1mg/ml BSA, PBS (Ca2+ and Mg< |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-HU 1 antibody, anti-MAPKAPK1A antibody, anti-Ribosomal protein S6 kinase 90kD 1 antibody, anti-Ribosomal protein S6 kinase 90kD polypeptide 1 antibody, anti-RSK 1 antibody, anti-RSK antibody, anti-RSK-1 antibody |
| ProteinTarget: | RSK1 p90 (phospho S363) |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Roux PP et al. Phosphorylation of p90 ribosomal S6 kinase (RSK) regulates extracellular signal-regulated kinase docking and RSK activity. Mol Cell Biol 23:4796-804 (2003). Cavet ME et al. 14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity. J Biol Chem 278:18376-83 (2003). Kim KW et al. Extracellular signal-regulated kinase/90-KDA ribosomal S6 kinase/nuclear factor-kappa B pathway mediates phorbol 12-myristate 13-acetate-induced megakaryocytic differentiation of K562 cells. J Biol Chem 276:13186-91 (2001). Richards SA et al. Characterization of regulatory events associated with membrane targeting of p90 ribosomal S6 kinase 1. Mol Cell Biol 21:7470-80 (2001). Zhang Y et al. UVA induces Ser381 phosphorylation of p90RSK/MAPKAP-K1 via ERK and JNK pathways. J Biol Chem 276:14572-80 (2001). Jensen CJ et al. 90-kDa ribosomal S6 kinase is phosphorylated and activated by 3-phosphoinositide-dependent protein kinase-1. J Biol Chem 274:27168-76 (1999). Smith JA et al. Identification of an extracellular signal-regulated kinase (ERK) docking site in ribosomal S6 kinase, a sequence critical for activation by ERK in vivo. J Biol Chem 274:2893-8 (1999). |
| Phosphorylated: | Ser363 |
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