| CatalogCode: | NSB906 |
| ProductName: | Dab1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Dab1 [Tyr198] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic phosphopeptide derived from the region of human Dab1 that contains tyrosine 198. |
| CrossReactivity: | Cross-reacts with Mouse. Expected to cross-react with Chicken (100% identity with immunogen), Rat (100% identity with immunogen), and human (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 ml protein A purified Rabbit antisera. |
| Uses: | WB: Use at a dilution of 1/1000. Detects a band of approximately 80 kDa. When examining phosphorylation of endogenous Dab1 protein we recommend that Dab1 protein first be immunoprecipitated. Recommended dilution is 1:1000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Control: | Human Embryonic Kidney (HEK293) cells transfected with wild-type mouse Dab1 protein. |
| Background: | Disabled 1 (Dab1) is an 80 kDa protein that is encoded by the Disabled 1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin. Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus. Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases (e.g., Fyn). Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1. Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3 beta (GSK-3 beta) and decreased phosphorylation of the microtubule-associated protein, Tau. Dab1 tyrosine 198 is a major site for reelin-induced Src family kinase-mediated phosphorylation in embryonic neurons. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium Azide Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Mu |
| ProteinTarget: | Dab1 phospho Y198 |
| PackageSize: | 0.1 ml |
| GeneralRef: | Arnaud L <i>et al.</i> Fyn tyrosine kinase is a critical regulator of disabled-1 during brain development. <i>Curr Biol</i> <b>13</b>:9-17 (2003). <PUBMED:12526739>Beffert U <i>et al.</i> Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta. <i>J Biol Chem</i> <b>277</b>:49958-64 (2002). <PUBMED:12376533>Keshvara L <i>et al.</i> Identification of reelin-induced sites of tyrosyl phosphorylation on disabled 1. <i>J Biol Chem</i> <b>276</b>:16008-14 (2001). <PUBMED:11279201>Howell BW <i>et al.</i> Dab1 tyrosine phosphorylation sites relay positional signals during mouse brain development. <i>Curr Biol</i> <b>10</b>:877-85 (2000). <PUBMED:10959835> 5. Hiesberger, T., et al. Direct binding of reelin to VLDL receptor and ApoE receptor 2 induces tyrosine phophorylation od disabled-1 and modulates tau phosphorylation. Nueron. 24(2): 481-489, 1999. 6. Homayouni, R., et al. Disabled-1 binds to the cytoplasmic domain of amylodi precursor-like protein 1. J. Neurosci. 19(17): 7507-7515, 1999. 7. Howell, B.W., et al. Reelin-induced tyrosine phosphorylation of disabled 1 during neuronal positioning. Genes Dev. 13(6): 643-648, 1999. |
| Phosphorylated: | Tyr198 |