| CatalogCode: | NSB900 |
| ProductName: | ErbB 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-ErbB 2 [Tyr1248] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic phosphopeptide derived from a region of human ErbB-2 that contains tyrosine 1248. |
| CrossReactivity: | Cross-reacts with Human and rat. Expected to cross-react with Dog and Hamster due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Use at a concentration of 0.5 - 1.0 ug/ml. Detects a band of approximately 185 kDa. IHC: 10-20 ug/ml (2 hr incubation)Not tested in other applications.**Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cell Membrane |
| Control: | SK-BR-3 cell line (overexpresses ErbB-2). |
| Background: | ErbB2 (also known as Her2 or neu) is a 185 kDa transmembrane receptor tyrosine kinase from the EGFR family that acts to regulate a variety of biological responses including cell growth, differentiation, and tissue development. Ligand binding to the extracellular domain, or overexpression of the receptor leads to phosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Overexpression or abnormal activation of ErbB2 has been found in a variety of tumors including brain, breast, lung and skin cancer. Tyrosine 1248 within the cytoplasmic domain of the receptor is an auto-phosphorylation site that allows binding of Shc and activation of the Ras, Raf, ERK1&2 signaling pathway. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1 |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: PBS, 1mg/ml BSA. pH 7.3 |
| ListPrice: | 310 |
| AppSummary: | WB, IHC |
| SpeciesSummary: | Hu, Rt |
| ALTnames: | anti-ErbB2 antibody, anti-ErbB-2 antibody, anti-c-erb B2 antibody, anti-v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 antibody, anti-HER2 antibody, anti-HER-2 antibody, anti-Neu antibody, anti-NGL antibody, anti-Neuroblastoma- or Glioblastoma-Derived antibody, anti-TKR1 antibody, anti-Tyrosine Kinase-type Cell Surface Receptor Her2 antibody |
| ProteinTarget: | ErbB 2 phospho Y1248 |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Dankort D et al. Grb2 and Shc adapter proteins play distinct roles in Neu (ErbB-2)-induced mammary tumorigenesis: implications for human breast cancer. Mol Cell Biol 21:1540-51 (2001). Lohrisch C & Piccart M An overview of HER2. Semin Oncol 28:3-11 (2001). Kwon YK et al. Activation of ErbB2 during wallerian degeneration of sciatic nerve. J Neurosci 17:8293-9 (1997). 3. DiGiovanna, Mp, & Stern, D.F. Activation state-spcific monoclonal antibody detects tyrosine phosphorylated p185neu/erbB-2 in a subset of human breast tumors overexpressing this receptor. Cancer Res. 55:1946-1955. 4. DiGiovanna, M.P., et al. Functional assay for HER-2/neu demonstrates active sinalling in a minority of HER-2/neu-overexpressing invasive human breast tumors. Brit. J. Cancer 74: 802-806. 5. DiGiovanna, M.P., et al. Active signaling by Neu in transgenic mice. Oncogene. 17:1877-1884, 1998. 6. Brandt, B.H., et al. c-erbB-2/EGFR as dominant heterodimerization partners determine a motogenic phenotype in human breast cancer cells. FASEB J. 13:1939-1949, 1999. |
| Phosphorylated: | Tyr1248 |
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