| CatalogCode: | NSB888 |
| ProductName: | Met (c-Met) Antibody |
| Product Description: | Rabbit Polyclonal anti-Met (c-Met) [Tyr1230/Tyr1234/Tyr1235] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic phosphopeptide derived from the region of human c-Met that contains tyrosines 1230, 1234 and 1235. |
| Specificity: | The phosphospecific antibody that has been generated does not distinguish between the dually (pYpY 1234/1235) and triply (pYpYpY1230/1234/1235) phosphorylated forms of c-Met, both of which are likely to represent activated forms of this receptor. |
| CrossReactivity: | Cross-reacts with Human and Mouse. Expected to cross-react with Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | Detects a band of approximately 169 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Type I membrane protein |
| Control: | 293T human kidney cells transiently transfected with human Met cDNA, stimulated with HGF.Mouse myelo |
| Background: | Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyr-xx- x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1) receptor and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of tyrosines 1234 and 1235 has been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, Erk1&2, JNK, PLC-ã, and STAT3). |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Constituents: 50% Glycerol, PBS, 1mg/ml BSA |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | Anti-c met antibody; Anti-cmet antibody; Anti-Hepatocyte growth factor receptor Precursor antibody; Anti-HGF receptor antibody; Anti-HGF SF receptor antibody; Anti-HGF/SF receptor antibody; Anti-HGFR antibody; Anti-Met proto oncogene tyrosine kinase antibody; Anti-Oncogene MET antibody; Anti-RCCP2 antibody; Anti-Renal cell carcinoma papillary 2 gene antibody; Anti-Scatter factor receptor antibody; Anti-SF receptor antibody |
| ProteinTarget: | Met (c-Met) phospho Y1230,1234,1235 |
| PackageSize: | 0.1 ml |
| GeneralRef: | <b>General / background references:</b>Vadnais J <i>et al.</i> Autocrine activation of the hepatocyte growth factor receptor/met tyrosine kinase induces tumor cell motility by regulating pseudopodial protrusion. <i>J Biol Chem</i> <b>277</b>:48342-50 (2002). <PUBMED:12372820>Fan S <i>et al.</i> The multisubstrate adapter Gab1 regulates hepatocyte growth factor (scatter factor)-c-Met signaling for cell survival and DNA repair. <i>Mol Cell Biol</i> <b>21</b>:4968-84 (2001). <PUBMED:11438654>Crostella L <i>et al.</i> Hepatocyte Growth Factor/scatter factor-induces phosphorylation of cortactin in A431 cells in a Src kinase-independent manner. <i>Oncogene</i> <b>20</b>:3735-45 (2001). <PUBMED:11439336>Parr C <i>et al.</i> The HGF/SF-induced phosphorylation of paxillin, matrix adhesion, and invasion of prostate cancer cells were suppressed by NK4, an HGF/SF variant. <i>Biochem Biophys Res Commun</i> <b>285</b>:1330-7 (2001). <PUBMED:11478803>5 5. Furge, K.A. et al. Met receptor tyrosine kinase:enhanced signaling through adapter protiens. Oncogene. 19(49):5582-5589, 2000. 6. Follenzi, A. et al. Cross-talk between the proto-oncogenes Met and Ron. Oncogene. 19(27):3041-3049, 2000. 7. Wei, L. et al. Expression, characterization, and crystallization of the catalytic core of the human insulin receptor protein-tyrosine kinase domain. J. Biol. Chem. 270(14):8122-8130, 1995. 8. Palka, H.L. et al. Hepatocyte growth factor receptor tyrosine kinase met is a substrate of the receptor ptoerin-tyrosine phosphatase DEP-1. J. Biol. Chem. 278(8):5728-5735, 2003. 9. Guiton, M. et al. Identification of in vivo brain-derived neurotrophic factor-stimulated autophosphorylation sites on the TrkB receptor tyrosine kinase by site-directed mutagenesis. J. Biol. Chem. 269(48):30370-30377, 1994. |
| Phosphorylated: | Tyr1230/Tyr1234/Tyr1235 |
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