| CatalogCode: | NSB882 |
| ProductName: | Met (c-Met) Antibody |
| Product Description: | Rabbit Polyclonal anti-Met (c-Met) [Tyr1003] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic phosphopeptide derived from the region of c-Met that contains tyrosine 1003. |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Detects a band of approximately 145 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Type I membrane protein |
| Control: | 293T cells transiently transfected with human c-Met and stimulated with HGF. |
| Background: | Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyr-xx- x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1R) and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of Tyr1234 and Tyr1235 have been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, ERK1&2, JNK1&2, PLC gamma, and STAT3). Tyr1003 is a negative regulatory phosphorylation site of c Met. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS [pH7.4] containing BSA (1 mg/mL) |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | Anti-c met antibody; Anti-cmet antibody; Anti-Hepatocyte growth factor receptor Precursor antibody; Anti-HGF receptor antibody; Anti-HGF SF receptor antibody; Anti-HGF/SF receptor antibody; Anti-HGFR antibody; Anti-Met proto oncogene tyrosine kinase antibody; Anti-Oncogene MET antibody; Anti-RCCP2 antibody; Anti-Renal cell carcinoma papillary 2 gene antibody; Anti-Scatter factor receptor antibody; Anti-SF receptor antibody |
| ProteinTarget: | Met (c-Met) phospho Y1003 |
| PackageSize: | 0.1 ml |
| GeneralRef: | Fan S et al. The multisubstrate adapter Gab1 regulates hepatocyte growth factor (scatter factor)-c-Met signaling for cell survival and DNA repair. Mol Cell Biol 21:4968-84 (2001). Crostella L et al. Hepatocyte Growth Factor/scatter factor-induces phosphorylation of cortactin in A431 cells in a Src kinase-independent manner. Oncogene 20:3735-45 (2001). Parr C et al. The HGF/SF-induced phosphorylation of paxillin, matrix adhesion, and invasion of prostate cancer cells were suppressed by NK4, an HGF/SF variant. Biochem Biophys Res Commun 285:1330-7 (2001). Longati P et al. Receptor tyrosine kinases as therapeutic targets: the model of the MET oncogene. Curr Drug Targets 2:41-55 (2001). Giordano S et al. Biosynthesis of the protein encoded by the c-met proto-oncogene. Oncogene 4:1383-8 (1989). |
| Phosphorylated: | Tyr1003 |
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