| CatalogCode: | NSB856 |
| ProductName: | Cortactin phospho Antibody |
| Product Description: | Rabbit Polyclonal anti-Cortactin phospho [Tyr466] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide derived from the region of mouse cortactin that contains tyrosine 466 (tyrosine 470 in the human sequence). |
| CrossReactivity: | Cross-reacts with Mouse. Expected to cross-react with Human (62% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Predicted molecular weight: 83 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | NIH3T3 cells expressing activated c-Src. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.4 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Mu |
| ProteinTarget: | Cortactin phospho |
| PackageSize: | 0.1 ml |
| GeneralRef: | <b>General / background references:</b>Dudek SM <i>et al.</i> Novel interaction of cortactin with endothelial cell myosin light chain kinase. <i>Biochem Biophys Res Commun</i> <b>298</b>:511-9 (2002). <PUBMED:12408982>Arderiu G <i>et al.</i> Primary arrest of circulating platelets on collagen involves phosphorylation of Syk, cortactin and focal adhesion kinase: studies under flow conditions. <i>Biochem J</i> <b>364</b>:65-71 (2002). <PUBMED:11988077>Weed SA & Parsons JT Cortactin: coupling membrane dynamics to cortical actin assembly. <i>Oncogene</i> <b>20</b>:6418-34 (2001). <PUBMED:11607842>Kapus A <i>et al.</i> Cell volume-dependent phosphorylation of proteins of the cortical cytoskeleton and cell-cell contact sites. The role of Fyn and FER kinases. <i>J Biol Chem</i> <b>275</b>:32289-98 (2000). <PUBMED:10921917> 5. Weed, S.A., et al. Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex. J. Cell Biol. 151(1): 29-40, 2000. 6. Huang, C., et al. The role of tyrosine phosphorylation of cortactin in the locomotion of endothelial cells. 273(40): 25770-25776, 1998. |
| Phosphorylated: | Tyr466 |
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