| CatalogCode: | NSB828 |
| ProductName: | IRS 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-IRS 2 [Ser731] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (YKASpSPAESS) corresponding to amino acids 727-736 of human IRS 2 at the phosphorylated site of Serine 731. This synthetic peptide also correlates to amino acids 129-138 of mouse IRS 2 at the phosphorylation sites of Serine 133 and to amino acids 229-238 of rat IRS 2 at the serinephosphorylation site 233. |
| Specificity: | This antibody is specific for human IRS 2 phosphorylated at the position of Serine 731. The antibodies were evaluated for specificity with a dot blot assay using synthetically prepared IRS peptides. It only recognizes the phosphorylated serine 731, not other phosphorylated sites or non-phosphorylated IRS 1 or IRS 2. |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.2 ml |
| Uses: | Immunoprecipitation: use at a concentration of 3.0 - 5.0 ug / extract from 10<sup>7</sup> cells.
Western Blotting: use at a concentration of 0.5 - 2.0 ug/ml.
ELISA: use at a concentration of 0.1 - 1.0 ug/ml.
Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects a band of approximately 200 kDa in cell lysate derived from mouse serum-treated fibroblasts, which corresponds to the predicted molecular weight of phospho IRS 2. Samples were boiled prior to subject 7.5% SDS-polyacrylamide gel electrophoresis. |
| Background: | The insulin receptor mediates insulin action through the phosphorylation of substrate proteins. The major substrates of the insulin receptor kinase are the insulin receptor substrate (IRS) family of proteins, IRS 1, IRS 2, IRS 3, IRS 4. IRS 2, a cytoplasmic signaling molecule that mediates effects of insulin, Insulin-like growthfactor 1 and other cytokines by acting as a molecular adaptor between diverse receptor kinases and downstream effectors. IRS proteins contain more than 70 potential Ser / Thr phosphorylation sites for kinases like PKA (cAMP dependent protein kinase), PKC, and MAPK. The phosphorylation of Ser / Thr residues of IRS proteins has adual function, serving either for a positive or negative modulation of insulin signal transduction. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS with 0.02% sodium azide |
| ListPrice: | 310 |
| AppSummary: | ELISA, IP, WB |
| SpeciesSummary: | Hu |
| ProteinTarget: | IRS 2 (phospho S731) |
| PackageSize: | 0.2 ml |
| GeneralRef: | 1. Liu YF et al. Insulin stimulates PKCzeta -mediated phosphorylation of insulin receptor substrate-1 (IRS-1). A self-attenuated mechanism to negatively regulate the function of IRS proteins. J Biol Chem 276:14459-65 (2001).
2. White MF The IRS-signalling system: a network of docking proteins that mediate insulin action. Mol Cell Biochem 182:3-11 (1998).
3. Lavan BE et al. The 60-kDa phosphotyrosine protein in insulin-treated adipocytes is a new member of the insulin receptor substrate family. J Biol Chem 272:11439-43 (1997).
4. Lavan BE et al. A novel 160-kDa phosphotyrosine protein in insulin-treated embryonic kidney cells is a new member of the insulin receptor substrate family. J Biol Chem 272:21403-7 (1997).
5. Mothe I & Van Obberghen E Phosphorylation of insulin receptor substrate-1 on multiple serine residues, 612, 632, 662, and 731, modulates insulin action. J Biol Chem 271:11222-7 (1996).
6. Sun XJ et al. Role of IRS-2 in insulin and cytokine signalling. Nature 377:173-7 (1995).
7. Sun XJ et al. Structure of the insulin receptor substrate IRS-1 defines a unique signal transduction protein. Nature 352:73-7 (1991).
8. Greene, M.W., et al. Modulation of insulin stimulated degredation of human insulin receptor substrate-1 by serine 312 phosphrylation. J. Biol. Chem. 278:8199-8211, 2003. |
| Phosphorylated: | Ser731 |
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