| CatalogCode: | NSB818 |
| ProductName: | IRS 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-IRS 1 [Tyr896] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide derived from a region of IRS 1 that contains tyrosine 896. The sequence is conserved in human, mouse, and rat. |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Predicted molecular weight: 165 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Control: | Chinese Hamster Ovary cell line transfected with a vector encoding the human insulin receptor (CHO-T |
| Background: | Insulin Receptor Substrate 1 (IRS 1) is a 165 kDa multi-phosphorylated cytoplasmic docking protein involved in the regulation of metabolism and proliferation by insulin, IL-4, and other cytokines. Insulin binding to the insulin receptor activates the catalytic domain and causes phosphorylation on tyrosine residues of IRS proteins, which in turn serve to recruit a variety of Src homology 2 (SH2) domain-containing proteins such as PI3 kinase, Grb 2, SHP 2, Nck and Crk. These proteins further propagate intracellular signalling, culminating in both metabolic and growth-promoting functions of IRS 1. pTyr896 of IRS 1 is a Grb 2 SH2 domain binding site. |
| Storage: | Store at -20 |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium Azide Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.4 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Insulin Receptor Substrate 1 antibody, anti-IRS 1 antibody anti-IRS1 antibody |
| ProteinTarget: | IRS 1 |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Aguirre V et al. Phosphorylation of Ser307 in insulin receptor substrate-1 blocks interactions with the insulin receptor and inhibits insulin action. J Biol Chem 277:1531-7 (2002). Hemi R et al. Transactivation of ErbB2 and ErbB3 by tumor necrosis factor-alpha and anisomycin leads to impaired insulin signaling through serine/threonine phosphorylation of IRS proteins. J Biol Chem 277:8961-9 (2002). Valverde AM et al. Association of insulin receptor substrate 1 (IRS-1) y895 with Grb-2 mediates the insulin signaling involved in IRS-1-deficient brown adipocyte mitogenesis. Mol Cell Biol 21:2269-80 (2001). Reiss K et al. Mechanisms of regulation of cell adhesion and motility by insulin receptor substrate-1 in prostate cancer cells. Oncogene 20:490-500 (2001). |
| Phosphorylated: | Tyr896 |