| CatalogCode: | NSB816 |
| ProductName: | IRS 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-IRS 1 [Tyr612] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide derived from a region of IRS 1 that contains tyrosine 612. The sequence is conserved in human, mouse, rat, and chicken. |
| Specificity: | IRS 2 (85% homologous) has also not been tested. |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Rat (100% identity with immunogen), Chicken (100% identity with immunogen), Mouse (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Use at a concentration of 1:1000 ug/ml. Predicted molecular weight: 165 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Control: | Chinese Hamster Ovary cell line transfected with a vector encoding the human insulin receptor (CHO-T |
| Background: | Insulin Receptor Substrate 1 (IRS 1) is a 165 kDa multi-phosphorylated cytoplasmic docking protein involved in the regulation of metabolism and proliferation by insulin, IL-4, and other cytokines. Insulin binding to the insulin receptor activates the catalytic domain and causes phosphorylation on tyrosine residues of IRS proteins, which in turn serve to recruit a variety of Src homology 2 (SH2) domain-containing proteins such as PI3 kinase, Grb 2, SHP 2, Nck and Crk. These proteins further propagate intracellular signalling, culminating in both metabolic and growth-promoting functions of IRS 1. pTyr612 of IRS 1 is in a pYMxM consensus motif PI3 kinase binding site. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.4 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Insulin Receptor Substrate 1 antibody, anti-IRS 1 antibody anti-IRS1 antibody |
| ProteinTarget: | IRS 1 |
| PackageSize: | 0.1 ml |
| GeneralRef: | <b>General / background references:</b>Shao J <i>et al.</i> Phosphatidylinositol 3-kinase redistribution is associated with skeletal muscle insulin resistance in gestational diabetes mellitus. <i>Diabetes</i> <b>51</b>:19-29 (2002). <PUBMED:11756318>Fujioka T <i>et al.</i> Further evidence for the involvement of insulin receptor substrates in epidermal growth factor-induced activation of phosphatidylinositol 3-kinase. <i>Eur J Biochem</i> <b>268</b>:4158-68 (2001). <PUBMED:11488908>Shaw LM Identification of insulin receptor substrate 1 (IRS-1) and IRS-2 as signaling intermediates in the alpha6beta4 integrin-dependent activation of phosphoinositide 3-OH kinase and promotion of invasion. <i>Mol Cell Biol</i> <b>21</b>:5082-93 (2001). <PUBMED:11438664>Esposito DL <i>et al.</i> Tyr(612) and Tyr(632) in human insulin receptor substrate-1 are important for full activation of insulin-stimulated phosphatidylinositol 3-kinase activity and translocation of GLUT4 in adipose cells. <i>Endocrinology</i> <b>142</b>:2833-40 (2001). <PUBMED:11416002> Hers, I. et al. Reciprocal feedback regulation of insulin receptor and insulin receptor substrate tyrosine phosphorylation by phosphoinositide 3-kinase in primary adipocytes. Biochem J. 368(3):875-884, 2002. Aguirre V. Phoshorylation of Ser307 in IRS-1 blocks interactions with the insulin receptor and inhibits insulin action. J. Biol. Chem. 277(2):1531-1537, 2002. Reiss, K. et al. Mechanisms of regulation of cell adhesion and motility by insulin receptor substrate-1 in prostrate cancer cells. Oncogene. 20:490-500, 2001. Ravichandran, L.V. et al. Protein kinase C-zeta phosphoylates insulin receptor substrate-1 and impairs its ability to activate phosphatidylinositol 3-kinase in response to insulin. J. Biol. Chem. 276(5):3543-3549, 2001. Li, J. et al. Modulation of insulin receptor substrate-1 tyrosine phosphorylation by Akt/phosphatidylinositol 3-kinase pathway. J. Biol. Chem. 274(14):9351-9356, 1999. Greene, M.W., et al. Modulation of human insulin receptor substrate-1 tyrosine phosphorylation by protein kinase C delta. J. Biochem. 378(1): 105-116, 2004. Kaiser, C., & James, S.R. Acetylation of insulin receptor substrate-1 is permissive for tyrosine phosphorylation. BMC. Biol. 2(1): 23-26, 2004. Reynoso, R., et al. High levels of palmitic acid lead to insulin resistance due to changes in the level of phosphorylation of the insulin receptor and insulin receptor substrate-1. Mol. Cell. Biochem. 246(1-2): 155-162, 2003. |
| Phosphorylated: | Tyr612 |
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