| CatalogCode: | NSB806 |
| ProductName: | Insulin Receptor Antibody |
| Product Description: | Rabbit Polyclonal anti-Insulin Receptor [Tyr1158/Tyr1162/Tyr1163] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor. The corresponding residues in the IGF1R are 1131, 1135 and 1136. |
| CrossReactivity: | Cross-reacts with Human and Mouse. Expected to cross-react with Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Use at a dilution of 1/1000. Detects a band of approximately 100 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Type I membrane protein |
| Control: | CHO-T cells transfected with a vector encoding the human insulin receptor and stimulated with insuli |
| Background: | Biological actions of insulin and IGF1 are mediated by their respective cell surface receptors, both of which are receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The Insulin Receptor and IGF1R are heterotetrameric proteins consisting of two ligand-binding alpha subunits and two beta subunits that each contain a tyrosine kinase domain. Insulin/IGF1 binding to the extracellular domain leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains. These differences allow insulin and IGF-1 to regulate different physiological functions through receptors that share a very similar structure. Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences. The catalytic loops within the tyrosine kinase domains of the Insulin Receptor/IGF1R contain a three tyrosine motif. It is generally believed that autophosphorylation within the activation loop proceeds in a processive manner initiating at the second tyrosine (1162 for the Insulin Receptor or 1135 for the IGF1R), followed by phosphorylation at the first tyrosine (1158 or 1131), then the last (1163 or 1136), upon which the Insulin Receptor or IGF1R becomes fully active. |
| Storage: | Store at -20 degrees Celcius. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20 degrees Celcius. For shipment or short-term storage (up to one week), 2-8 degrees Celcius. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium Azide Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-IGFR1 antibody, anti-Insulin growth factor 1 receptor antibody, anti-CD221 antibody |
| ProteinTarget: | Insulin Receptor / IGF 1 Receptor phospho Y1158 + Y1162 + Y1163 |
| PackageSize: | 0.1 ml |
| GeneralRef: | This product has been used in:Pender C et al. Regulation of insulin receptor function by a small molecule insulin receptor activator. J Biol Chem 277:43565-71 (2002). <PUBMED:12213804>General / background references:Bevan P Insulin signalling. J Cell Sci 114:1429-30 (2001). <PUBMED:11282018>Skorey KI et al. Development of a robust scintillation proximity assay for protein tyrosine phosphatase 1B using the catalytically inactive (C215S) mutant. Annual Biochem 291:269-78 (2001). <PUBMED:11401301>Ebina Y et al. The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell 40:747-58 (1985). <PUBMED:2859121> 4. Ottensmeyer, F.P., et al. Mechanism of transmembrance signaling:insulin binding and the insulin receptor. Biochem. 39(40): 12103-12112, 2000. 5. Playford, M.P., et al. Insulin-like growth factor 1 regulates the location, stability, and transcriptional activity of beta-catenin. PNAS. 97(22): 12103-12108. 6. Ullrich, A., et al. Human insulin receptor and tis relationship to the tyrosine kinase family of oncogenes. Nature. 313(6005): 756-761. |
| Phosphorylated: | Tyr1158/Tyr1162/Tyr1163 |
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