| CatalogCode: | NSB728 |
| ProductName: | eIF-2A Antibody |
| Product Description: | Rabbit Polyclonal anti-eIF-2A [Ser52] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (Human) derived from the region of human eIF-2 alpha that contains serine 52. This region is conserved among many species including rat, pig, cow, fruit fly, and yeast. |
| Epitope: | KLH-conjugated, synthetic peptide (SEL[pS]RRRIRSINK) corresponding to amino acids 48-60 of Drosophila eIF-2á. |
| CrossReactivity: | Cross-reacts with Human, Mouse and S. cerevisiae. Expected to cross-react with a wide range of other species due to sequence homology (100%). Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Use at a dilution of 1/1000. Predicted molecular weight: 36 kDa. Can be blocked with <ab5243>eIF-2A phospho S51 and non-phospho peptides (pair)</ab>Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Control: | 3T3-L1 adipocytes stimulated with Leukemia Inhibitory Factor (LIF). |
| Background: | eIF2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF2 alpha is phosphorylated at serine 51 (human EIF2 alpha, the equivalent residue in mouse is serine 52) by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important regulatory process in protein synthesis. |
| ProductRef: | 1. Brush, M.H., et al. Growth arrest and DNA damage-inducible protein GADD34 targets protein phosphatease 1 alpha to the endoplasmic reticulum and promotes dephosphorylation of the alpha subunit of eukaryotic translation initiation factor 2. Mol. Cell Biol. 23(4):1292-1303, 2003. 2. Kimball, S.R., et al. Mammalian stress granules represent sites of accumulation of stalled translocation initiation complexes. Am. J. Physiol. Cell Physiol. 284(2):C273-C284, 2003. 3. Singleton, R.H., et al. Traumatically induced axotomy adjacent to the soma does not result in acute neuronal death. J. Neurosci. 22(3):791-802, 2002. 4. Pataer A et al. Adenoviral transfer of the melanoma differentiation-associated gene 7 (mda7) induces apoptosis of lung cancer cells via up-regulation of the double-stranded RNA-dependent protein kinase (PKR). Cancer Res 62:2239-43 (2002). 5. Soboloff J & Berger SA Sustained ER Ca2+ depletion suppresses protein synthesis and induces activation-enhanced cell death in mast cells. J Biol Chem 277:13812-20 (2002). 6. Grundmann O et al. Repression of GCN4 mRNA translation by nitrogen starvation in Saccharomyces cerevisiae. J Biol Chem 276:25661-71 (2001). Li S & Koromilas AE Dominant negative function by an alternatively spliced form of the interferon-inducible protein kinase PKR. J Biol Chem 276:13881-90 (2001). 7. Kumar R et al. Brain ischemia and reperfusion activates the eukaryotic initiation factor 2alpha kinase, PERK. J Neurochem 77:1418-21 (2001). 8. Patel CV et al. PACT, a stress-modulated cellular activator of interferon-induced double-stranded RNA-activated protein kinase, PKR. J Biol Chem 275:37993-8 (2000). 9. Vorburger, S.A., et al. Role for the double-stranded RNA activated protein kiase PKR in E2F-1-induced apoptosis. Oncogene. 21(41):6278-6288, 2002. 10. Zhang, P., et al. The PERK eukaryotic initiation factor 2 alpha kinase is required for the development of the skeletal system, postnatal growth, and the function and viability of the pancreas. Mol. Cell. Biol. 22(11): 3864-3874, 2002. 11. Lu, L., et al. Translation initiation control by heme-regulated eukaryotic initiation factor 2alpha kinase in erythoid cells under cytoplasmic stresses. Mol. Cell. Biol. 21(23):7971-7980, 2001. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu, Ye |
| ALTnames: | anti-Eukaryotic Translation Initiation Factor 2 alpha antibody, anti-CDA 02 antibody, anti-CDA02 antibody, anti-EIF-2alpha antibody, anti-eIF2S1 antibody |
| ProteinTarget: | eIF-2A phospho |
| PackageSize: | 0.1 ml |
| GeneralRef: | b>Pataer A et al. Adenoviral transfer of the melanoma differentiation-associated gene 7 (mda7) induces apoptosis of lung cancer cells via up-regulation of the double-stranded RNA-dependent protein kinase (PKR). Cancer Res 62:2239-43 (2002). Soboloff J & Berger SA Sustained ER Ca2+ depletion suppresses protein synthesis and induces activation-enhanced cell death in mast cells. J Biol Chem 277:13812-20 (2002). Grundmann O et al. Repression of GCN4 mRNA translation by nitrogen starvation in Saccharomyces cerevisiae. J Biol Chem 276:25661-71 (2001). Li S & Koromilas AE Dominant negative function by an alternatively spliced form of the interferon-inducible protein kinase PKR. J Biol Chem 276:13881-90 (2001). Kumar R et al. Brain ischemia and reperfusion activates the eukaryotic initiation factor 2alpha kinase, PERK. J Neurochem 77:1418-21 (2001). Patel CV et al. PACT, a stress-modulated cellular activator of interferon-induced double-stranded RNA-activated protein kinase, PKR. J Biol Chem 275:37993-8 (2000). |
| Phosphorylated: | Ser52 |
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