| CatalogCode: | | NSB726 |
| ProductName: | | IKB alpha Antibody |
| Product Description: | | Rabbit Polyclonal anti-IKB alpha [Ser32/Ser36] |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic phosphopeptide derived from the region of human IKBa that contains serines 32 and 36. |
| CrossReactivity: | | Cross-reacts with Human and Mouse (see review). Expected to cross-react with Chicken (93% identity with immunogen), Pig (100% identity with immunogen), Rat (93% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | | WB: Use at a dilution of 1/1000. Detects a band of approximately 40 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | | Cytoplasmic |
| Control: | | Jurkat cells pretreated for 30 minutes with ALLN and stimulated with TNF-á. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | Preservative: 0.05% Sodium Azide Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
| ListPrice: | | 325 |
| AppSummary: | | WB |
| SpeciesSummary: | | Hu |
| ProteinTarget: | | IKB alpha phospho S32 / 36 |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | <b>General / background references:</b>Ding GR <i>et al.</i> Radiosensitization by inhibition of IkappaB-alpha phosphorylation in human glioma cells. <i>Radiat Res</i> <b>160</b>:232-7 (2003). <PUBMED:12859235>Miskolci V <i>et al.</i> Okadaic acid induces sustained activation of NFkappaB and degradation of the nuclear IkappaBalpha in human neutrophils. <i>Arch Biochem Biophys</i> <b>417</b>:44-52 (2003). <PUBMED:12921778>Yang L <i>et al.</i> RelA control of IkappaBalpha phosphorylation: a positive feedback loop for high affinity NF-kappaB complexes. <i>J Biol Chem</i> <b>278</b>:30881-8 (2003). <PUBMED:12663663>Chen F <i>et al.</i> New insights into the role of nuclear factor-kappaB in cell growth regulation. <i>Am J Pathol</i> <b>159</b>:387-97 (2001). <PUBMED:11485895> 4. Ghosh, S., & Karin, M. Missing pieces of the NF-kappaB puzzle. Cell. 109(supp): S81-S96, 2002. 5. Carcamo, J.M., et al. Vitamin A suppresses TNFalpha-induced NF-kappaB activation by inhibiting IkappaBalpha phosphorylation. Biochem. 4(43): 12995-13002, 2002. 6. Pandey, S.K., et al. Wortmannin-sensitive pathway is required for insulin-stimulated phosphorylation of inhibitor kappaBalpha. Endocrin. 143(2): 375-385, 2002. 7. DiDonato, J., et al. Mapping of the inducible IkappaB phosphorylation sites that signal its ubiquitination and degredation. Mol. Cell Bio. 16(4): 1295-1304, 1997. 8. Li, X., et al. Chracterization of NFkappaB activation by detection of green fluorescent protein tagged IkappaB degredation in living cells. J. Biol. Chem. 274(30): 21244-21250, 1999. |
| Phosphorylated: | | Ser32/Ser36 |
| more information: | | company product webpage |