| CatalogCode: | NSB722 |
| ProductName: | Paxillin Antibody |
| Product Description: | Rabbit Polyclonal anti-Paxillin [Tyr118] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (Human) derived from the region of human paxillin that contains tyrosine 118. |
| CrossReactivity: | Cross-reacts with Human and Mouse.Expected to cross-react with Chicken (87% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: Use at a concentration of 0.5 - 1.0 ug/ml. Predicted molecular weight: 68 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | NMuMG cells transfected with EGFP-tagged paxillin. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-FLJ23042 antibody, anti-PXN antibody |
| ProteinTarget: | Paxillin |
| PackageSize: | 0.1 ml |
| GeneralRef: | This product has been used in:Manabe Ri R et al. GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. J Cell Sci 115:1497-510 (2002). Nikolopoulos SN & Turner CE Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization. J Biol Chem 277:1568-75 (2002). van de Water B et al. Suppression of chemically induced apoptosis but not necrosis of renal proximal tubular epithelial (LLC-PK1) cells by focal adhesion kinase (FAK). Role of FAK in maintaining focal adhesion organization after acute renal cell injury. J Biol Chem 276:36183-93 (2001). Weinberg JM et al. Energetic determinants of tyrosine phosphorylation of focal adhesion proteins during hypoxia/reoxygenation of kidney proximal tubules. Am J Pathol 158:2153-64 (2001). West KA et al. The LD4 motif of paxillin regulates cell spreading and motility through an interaction with paxillin kinase linker (PKL). J Cell Biol 154:161-76 (2001). Schaller MD & Schaefer EM Multiple stimuli induce tyrosine phosphorylation of the Crk-binding sites of paxillin. Biochem J 360:57-66 (2001). Nakamura K et al. Tyrosine phosphorylation of paxillin alpha is involved in temporospatial regulation of paxillin-containing focal adhesion formation and F-actin organization in motile cells. J Biol Chem 275:27155-64 (2000). |
| Phosphorylated: | Tyr118 |