| CatalogCode: | NSB711 |
| ProductName: | NFkB p50 Antibody |
| Product Description: | Rabbit Polyclonal anti-NFkB p50 [Ser529] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (Human). |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Mouse (91% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Use at a dilution of 1/1000. Detects a band of approximately 60 kDa. |
| Localization: | Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I kappa B). |
| Background: | p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium Azide Constituents: 50% Glycerol, 1.0mg/ml BSA, PBS (Ca<sup>2+</ |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-DKFZp686C01211 antibody, anti-DNA binding factor KBF1 antibody, anti-DNA binding factor KBF1 EBP1 antibody, anti-EBP 1 antibody, anti-EBP1 antibody, anti-KBF1 antibody, anti-MGC54151 antibody, anti-NF kappa B antibody, anti-NFKB 1 antibody, anti-NFKB p105 antibody, anti-NFKB p50 antibody, anti-NFKB1 antibody, anti-Nuclear factor kappa B DNA binding subunit antibody, anti-Nuclear factor NF kappa B p105 subunit antibody, anti-Nuclear factor NF kappa B p50 subunit antibody, anti-Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody |
| ProteinTarget: | NFkB p50 (phospho S529) |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Haller D et al. IKK beta and phosphatidylinositol 3-kinase/Akt participate in non-pathogenic Gram-negative enteric bacteria-induced RelA phosphorylation and NF-kappa B activation in both primary and intestinal epithelial cell lines. J Biol Chem 277:38168-78 (2002). Hunter RB et al. Activation of an alternative NF-kappaB pathway in skeletal muscle during disuse atrophy. FASEB J 16:529-38 (2002). Martin AG et al. Regulation of nuclear factor kappa B transactivation. Implication of phosphatidylinositol 3-kinase and protein kinase C zeta in c-Rel activation by tumor necrosis factor alpha. J Biol Chem 276:15840-9 (2001). Madrid LV et al. Akt stimulates the transactivation potential of the RelA/p65 Subunit of NF-kappa B through utilization of the Ikappa B kinase and activation of the mitogen-activated protein kinase p38. J Biol Chem 276:18934-40 (2001). Wang D et al. Tumor necrosis factor alpha-induced phosphorylation of RelA/p65 on Ser529 is controlled by casein kinase II. J Biol Chem 275:32592-7 (2000). Wang D & Baldwin AS Activation of nuclear factor-kappaB-dependent transcription by tumor necrosis factor-alpha is mediated through phosphorylation of RelA/p65 on serine 529. J Biol Chem 273:29411-6 (1998). |
| Phosphorylated: | Ser529 |
|