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| Catalog Code: | NSB699 |
| Product Name: | PARP Antibody |
| Product Description: | Rabbit Polyclonal anti-PARP, conjugated to FITC |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human) corresponding to N-terminus of cleavage site (214/215) of human PARP. |
| Specificity: | This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells. |
| Cross Reactivity: | Cross-reacts with Human and Cow. Not yet tested in other species. |
| Packaging: | 1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | FACS: Use at an assay dependant dilution. The unconjugated antibody to cleaved PARP has been used successfully for Western blotting and immunohistochemistry applications. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates the N-terminal DNA binding domain (24 kDa) from the C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Constituents: PBS, BSA. pH 7.2 |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Bv, Hu, |
| Alternate Names: | anti-ADP ribosyltransferase NAD+ antibody, anti-pADPRT1 antibody, anti-PARP1 antibody, anti-Poly adenosine diphosphate ADP ribose polymerase antibody, anti-Poly ADP ribose polymerase 1 antibody, anti-Poly ADP ribose synthetase 1 antibody, anti-PPOL antibody |
| Package Size: | 1 ml |
| Preservative: | 0.1% Sodium Azide |
| Conjugate: | FITC |
| General References: | </b>Germain M <i>et al.</i> Cleavage of automodified poly(ADP-ribose) polymerase during apoptosis. Evidence for involvement of caspase-7. <i>J Biol Chem</i> <b>274</b>:28379-84 (1999). <PUBMED:10497198>Duriez PJ & Shah GM Cleavage of poly(ADP-ribose) polymerase: a sensitive parameter to study cell death. <i>Biochem Cell Biol</i> <b>75</b>:337-49 (1997). <PUBMED:9493956>Tewari M <i>et al.</i> Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. <i>Cell</i> <b>81</b>:801-9 (1995). <PUBMED:7774019>Kaufmann SH <i>et al.</i> Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis. <i>Cancer Res</i> <b>53</b>:3976-85 (1993). <PUBMED:8358726> 5. Kumar, A.P., et al. 2-Methoxyestradiol blocks cell-cycle progession at G(2)/M phase and inhibits growth of human prostrate cancer cells. Mol. Carcinogenesis. 3:111-124, 2001. |
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