| CatalogCode: | NSB696 |
| ProductName: | Phospholipase C gamma-1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Phospholipase C gamma-1 [Tyr783] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human PLCg-1 that contains tyrosine 783. This region is conserved among many species including mouse, rat, cow and frog. |
| CrossReactivity: | Human and mouse PLCg-1. Other species (100% homologous) have not been tested, but are expected to react. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody is suitable for use in Western blotting. The recommended concentration for use in Western blotting is 1:1,000. *The optimal concentration should be determined for each specific application. |
| Control: | NIH3T3 cells +/- PDGF. |
| Background: | Phosphoinositide-specific phospholipase C (PLC) is a family of proteins that hydrolyze phosphatidylinositol 4,5-bisphosphate to generate the second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). IP3 induces a transient increase in intracellular free Ca++, while DAG is a direct activator of protein kinase C (PKC). These processes are involved in the control of multiple cellular activities such as cell proliferation, growth and differentiation. PLCg-1 is a 135 kDa ubiquitously expressed isozyme that is activated by phosphorylation of tyrosine 783 and interacts with growth factor receptors (EGFR, PDGFR, etc.) and adhesion/cytoskeletal proteins (FAK, paxillin) to influence a multiple number of signaling pathways. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Host_Name: | Rabbit |
| Buffer: | 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-PLCG 1 antibody |
| ProteinTarget: | Phospholipase C gamma-1 Phosphospecific |
| PackageSize: | 0.1 ml |
| GeneralRef: | Meyer, R.D., et al. (2003) Recruitment and activation of phospholipase Cgamma1 by vascular endothelial growth factor receptor-2 are required for tubulogenesis and differentiation of endothelial cells. J. Biol. Chem. 278(18):16347-16355 (cites the use of this antibody). Arbiser, J.L., et al. (2002) Functional tyrosine kinase inhibitor profiling: a generally applicable method points to a novel role of platelet-derived growth factor receptor-beta in tuberous sclerosis. Am. J. Pathol. 161(3):781-786 (cites the use of this antibody). Cho, S.Y. and R.L. Klemke (2002) Purification of pseudopodia from polarized cells reveals redistribution and activation of Rac through assembly of a CAS/Crk scaffold. J. Cell Biol. 156(4):725-736 (cites the use of cat. # 44-614, 44-624, 44-652 and 44-696). Meyer, R.D., et al. (2002) The presence of a single tyrosine residue at the carboxyl domain of vascular endothelial growth factor receptor-2/FLK-1 regulates its autophosphorylation and activation of signaling molecules. J. Biol. Chem. 277(30):27081-27087 (cites the use of cat. # 44-660 and 44-696). Reynolds, L.F., et al. (2002) Vav1 transduces T cell receptor signals to the activation of phospholipase C-gamma1 via phosphoinositide 3-kinase-dependent and -independent pathways. J. Exp. Med. 195(9):1103-1114 (cites the use of this antibody). Dayanir, V., et al. (2001) Identification of tyrosine residues in vascular endothelial growth factor receptor-2/FLK-1 involved in activation of phosphatidylinositol 3-kinase and cell proliferation. J. Biol. Chem. 276(21):17686-17692 (cites the use of this antibody). Manzerra, P., et al. (2001) Zinc induces a Src family kinase-mediated up-regulation of NMDA receptor activity and excitotoxicity. Proc. Nat'l. Acad. Sci. USA 98(20):11055-11061 (cites the use of cat. # 44-658, 44-660, 44-662 and 44-696). Rebecchi, M.J. and S.N. Pentyala (2000) Structure, function, and control of phosphoinositide-specific phospholipase C. Physiol. Rev. 80(4):1291-1335.Zhang, X., et al. (1999) Focal adhesion kinase promotes PLCg-1 activity Proc. Nat'l. Acad. Sci. USA 96(16):9021-9026. Lockhart, L.K. and A. McNicol (1999) The PLC inhibitor U73122 inhibits phorbol ester-induced platelet activation. J. Pharmacol. Exp. Ther. 289(2):721-728. Assefa, Z., et al. (1999) JNK/SAPK activation by platelet-derived growth factor in A431 cells requires both the PLCg and the phosphatidylinositol 3-kinase signaling pathways of the receptor. Biochem. Biophys. Res. Commun. 261(3):641-645. |
| Phosphorylated: | Tyr783 |
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