| CatalogCode: | NSB662 |
| ProductName: | Src Antibody |
| Product Description: | Rabbit Polyclonal anti-Src [Tyr529] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (Human) derived from the region of Src that contains tyrosine 529, based on the human sequence. The sequence is conserved in mouse (tyrosine 534), chicken (tyrosine 527) and frog (tyrosine 525). |
| Specificity: | Fyn and Yes (92%homologous) were not tested. |
| CrossReactivity: | Cross-reacts with Human, Chicken, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | IHC: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Predicted molecular weight: 62 kDa. Can be blocked with <ab5257>Src phospho Y529 and non-phospho peptides (pair)</ab> or <ab5353>CEF whole cell lysate, positive / negative for Src (pair)</ab>Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Control: | Chicken Embryo Fibroblast (CEF) cells expressing Src protein and plated on fibronectin. |
| Background: | Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of the catalytic domain residue (tyrosine 418 in the human sequence), thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylatedleading to full activation. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
| ListPrice: | 325 |
| AppSummary: | IHC, WB |
| SpeciesSummary: | Ch, Hu, Mu, Rt |
| ALTnames: | anti-ASV antibody, anti-cSrc antibody, anti-Neuronal CSRC tyrosine specific protein kinase antibody, anti-p60Src antibody, anti-pp60csrc antibody, anti-protooncogene SRC Rous sarcoma antibody, anti-Tyrosine kinase pp60c src antibody, anti-Tyrosine protein kinase SRC1 antibody, anti-vsrc avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody |
| ProteinTarget: | Src |
| PackageSize: | 0.1 ml |
| GeneralRef: | <b>This product has been used in:</b>Haas M <i>et al.</i> Src-mediated inter-receptor cross-talk between the Na+/K+-ATPase and the epidermal growth factor receptor relays the signal from ouabain to mitogen-activated protein kinases. <i>J Biol Chem</i> <b>277</b>:18694-702 (2002). <PUBMED:11907028>Suhr SM <i>et al.</i> Antisense oligodeoxynucleotide evidence that a unique osteoclastic protein-tyrosine phosphatase is essential for osteoclastic resorption. <i>J Bone Miner Res</i> <b>16</b>:1795-803 (2001). <PUBMED:11585343>Gil-Henn H <i>et al.</i> Regulation of protein-tyrosine phosphatases alpha and epsilon by calpain-mediated proteolytic cleavage. <i>J Biol Chem</i> <b>276</b>:31772-9 (2001). <PUBMED:11429406>Cheng A <i>et al.</i> Attenuation of adhesion-dependent signaling and cell spreading in transformed fibroblasts lacking protein tyrosine phosphatase-1B. <i>J Biol Chem</i> <b>276</b>:25848-55 (2001). <PUBMED:11346638>Giusti AF <i>et al.</i> Evidence that fertilization activates starfish eggs by sequential activation of a Src-like kinase and phospholipase cgamma. <i>J Biol Chem</i> <b>275</b>:16788-94 (2000). <PUBMED:10747984>Ma YC <i>et al.</i> Src tyrosine kinase is a novel direct effector of G proteins. <i>Cell</i> <b>102</b>:635-46 (2000). <PUBMED:11007482>Sussman MA <i>et al.</i> Altered focal adhesion regulation correlates with cardiomyopathy in mice expressing constitutively active rac1. <i>J Clin Invest</i> <b>105</b>:875-86 (2000). <PUBMED:10749567>Maher PA Disruption of cell-substrate adhesion activates the protein tyrosine kinase pp60(c-src). <i>Exp Cell Res</i> <b>260</b>:189-98 (2000). <PUBMED:11035913>Osusky M <i>et al.</i> Autophosphorylation of purified c-Src at its primary negative regulation site. <i>J Biol Chem</i> <b>270</b>:25729-32 (1995). <PUBMED:7592753> Bock, H.H. & Herz, J. Reelin activates SRC family tyrosine kinases in neurons. Curr. Bio. 13(1):18-26, 2003. Pankov, R. et al. Specific beta1 integrin site selectively regulates Akt/prtoein kinase B signaling via local activation of protein phosphotase 2A. J. Biol. Chem. 277(21):18694-18702, 2003. Lacalle, R.A. et al Specific SHP-2 partitioning in raft domains triggers integrin-medicated signaling via Rho activation. J. Cell Bio. 157(2):277-289, 2002. Obergfell, A. et al. Coordinate interactions of Csk, Src, and Syk kinases with aIIbBeta3 initiate integrin signaling to the cytoskeleton. J. Cell Bio. 157(2):265-275, 2002. Manzerra, P. et al. Zinc induces a Src family kinase-medicated up-regulation of NMDA receptor activity and excitoxicity. PNAS. 98(20);11055-11061, 2001. |
| Phosphorylated: | Tyr529 |
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