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Rabbit Polyclonal anti-PYK 2 [Tyr850]
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CatalogCode:NSB634
ProductName:PYK 2 Antibody
Product Description:Rabbit Polyclonal anti-PYK 2 [Tyr850]
Clonality:Polyclonal
Immunogen:Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosine 580. The sequence is conserved in human and rat.
CrossReactivity:Cross-reacts with Human, Chicken and Rat.Not yet tested in other species.
Packaging:0.1 ml
Uses:WB: Use at a concentration of 0.1 - 1.0 ug/ml. Predicted molecular weight: 125 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Background:Proline-rich/Ca2+ -activated tyrosine kinase (PYK 2) is a member of the Focal Adhesion Kinase family of non-receptor, proline-rich PTK's. PYK 2 signaling is initiated by a variety of extracellular stimuli including integrin ligation, CD surface marker ligation (e.g., CD3, CD28, TCR, VCAM), bioactive peptides, growth factors, cytokines, chemokines, and certain stress stimuli. PYK 2 is involved in the regulation of vesicular transport, osteoclastic bone resorption, modulation of ion channels, T- and B-cell receptor signaling and cell death. Phosphorylation of the catalytic domain tyrosines 579 and 580 results in maximum activation of PYK 2. pTyr579 and pTyr580 are phosphorylated by Src.
Purity:Immunogen affinity purified
Isotype:IgG
Host_Name:Rabbit
Buffer:Preservative: NoneConstituents: 50% Glycerol, PBS. pH 7.4
ListPrice:325
AppSummary:WB
SpeciesSummary:Hu, Ch, Rt
ALTnames:anti-FADK antibody, anti-FAK 2 antibody, anti-FAK antibody, anti-FAK1 antibody, anti-Focal Adhesion Kinase 2 antibody, anti-pp125FAK antibody, anti-Proline Rich Tyrosine Kinase 2 antibody, anti-Protein Tyrosine Kinase 2 Beta antibody, anti-PTK 2B antibody
ProteinTarget:PYK 2
PackageSize:0.1 ml
GeneralRef:This product has been used in:Nakamura K et al. Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies. Oncogene 20:2626-35 (2001). Sorokin A et al. Protein-tyrosine kinase Pyk2 mediates endothelin-induced p38 MAPK activation in glomerular mesangial cells. J Biol Chem 276:21521-8 (2001). Katagiri T et al. Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402. J Biol Chem 275:19645-52 (2000). Keely SJ et al. Carbachol-stimulated transactivation of epidermal growth factor receptor and mitogen-activated protein kinase in T(84) cells is mediated by intracellular ca(2+), PYK-2, and p60(src). J Biol Chem 275:12619-25 (2000). Shi CS et al. G13alpha-mediated PYK2 activation. PYK2 is a mediator of G13alpha -induced serum response element-dependent transcription. J Biol Chem 275:24470-6 (2000).
Phosphorylated:Tyr850
see all human PTK2B antibodies
see anti rabbit secondary antibodies


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