| CatalogCode: | NSB618 |
| ProductName: | PYK 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-PYK 2 [Tyr402] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosine 402. The sequence is conserved in human and rat. |
| CrossReactivity: | Cross-reacts with Human, Chicken and Rat.Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | IHC: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Predicted molecular weight: 116 kDa. Can be blocked with <ab5312>PYK 2 phospho Y402 and non-phospho peptides (pair)</ab> or <ab5352>CEF whole cell lysate, positive / negative for PYK 2 (pair)</ab>Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Control: | Chick embryo fibroblast (CEF) cells expressing human PYK 2. |
| Background: | Proline-rich/Ca2+ -activated tyrosine kinase (PYK 2) is a member of the Focal Adhesion Kinase family of non-receptor, proline-rich PTK's. PYK 2 signaling is initiated by a variety of extracellular stimuli including integrin ligation, CD surface marker ligation (e.g., CD3, CD28, TCR, VCAM), bioactive peptides, growth factors, cytokines, chemokines, and certain stress stimuli. PYK 2 is involved in the regulation of vesicular transport, osteoclastic bone resorption, modulation of ion channels, T- and B-cell receptor signaling and cell death. pTyr402 is the autophosphorylation site of PYK 2. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH |
| ListPrice: | 325 |
| AppSummary: | IHC, WB |
| SpeciesSummary: | Hu, Ch, Rt |
| ALTnames: | anti-FADK antibody, anti-FAK 2 antibody, anti-FAK antibody, anti-FAK1 antibody, anti-Focal Adhesion Kinase 2 antibody, anti-pp125FAK antibody, anti-Proline Rich Tyrosine Kinase 2 antibody, anti-Protein Tyrosine Kinase 2 Beta antibody, anti-PTK 2B antibody |
| ProteinTarget: | PYK 2 |
| PackageSize: | 0.1 ml |
| GeneralRef: | This product has been used in:Sajan MP et al. Sorbitol activates atypical protein kinase C and GLUT4 glucose transporter translocation/glucose transport through proline-rich tyrosine kinase-2, the extracellular signal-regulated kinase pathway and phospholipase D. Biochem J 362:665-74 (2002). Sorokin A et al. Protein-tyrosine kinase Pyk2 mediates endothelin-induced p38 MAPK activation in glomerular mesangial cells. J Biol Chem 276:21521-8 (2001). Nakamura K et al. Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies. Oncogene 20:2626-35 (2001). Katagiri T et al. Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402. J Biol Chem 275:19645-52 (2000). Shi CS et al. G13alpha-mediated PYK2 activation. PYK2 is a mediator of G13alpha -induced serum response element-dependent transcription. J Biol Chem 275:24470-6 (2000). Keely SJ et al. Carbachol-stimulated transactivation of epidermal growth factor receptor and mitogen-activated protein kinase in T(84) cells is mediated by intracellular ca(2+), PYK-2, and p60(src). J Biol Chem 275:12619-25 (2000). |
| Phosphorylated: | Tyr402 |
order or
more information: | company product webpage |
| request information: |
|
|