| CatalogCode: | | NSB604 |
| ProductName: | | GSK 3 (alpha + beta) Antibody |
| Product Description: | | Rabbit Polyclonal anti-GSK 3 (alpha + beta) [Tyr216/Tyr279] |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide derived from the regions of GSK 3 alpha + beta protein that contain tyrosine 216/279. |
| Specificity: | | Phosphorylation site-specific antibody selective for the phosphorylated isoforms of glycogen synthase kinase enzyme, GSK 3 alpha, a 51 kDa protein containing a phosphate on tyrosine 279, and GSK 3 beta, a 47 kDa protein containing a phosphate on tyrosine 216. |
| CrossReactivity: | | Cross-reacts with Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | | Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. WB: Use at a concentration of 0.5 - 1.0 ug/ml. Predicted molecular weight: 51kDa for GSK 3 alpha and 47 kDa for GSK 3 beta. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Control: | | Serum starve 3T3-L1 cells for 3 hours, then stimulate with 100 nM insulin or 100 ng/mL IGF 1 for 5 m |
| Background: | | Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | Preservative: None Constituents: PBS. pH 7.4 |
| ListPrice: | | 325 |
| AppSummary: | | ELISA, WB |
| SpeciesSummary: | | Hu, Mu, Rt |
| ALTnames: | | anti-C86142 antibody, anti-FA antibody, anti-Factor A antibody, anti-Glycogen Synthase Kinase 3 Alpha and Beta antibody, anti-Glycogen synthase kinase 3 alpha antibody, anti-Glycogen synthase kinase 3 beta antibody, anti-GSK 3 alpha antibody, anti-GSK 3 beta antibody, anti-Gsk3a antibody, anti-KG3A antibody, anti-KG3B antibody |
| ProteinTarget: | | GSK 3 (alpha + beta) |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | This product has been used in:Papasozomenos SCh & Shanavas A Testosterone prevents the heat shock-induced overactivation of glycogen synthase kinase-3 beta but not of cyclin-dependent kinase 5 and c-Jun NH2-terminal kinase and concomitantly abolishes hyperphosphorylation of tau: implications for Alzheimer's disease. Proc Natl Acad Sci U S A 99:1140-5 (2002). Kirschenbaum F et al. Substitution of a glycogen synthase kinase-3beta phosphorylation site in presenilin 1 separates presenilin function from beta-catenin signaling. J Biol Chem 276:7366-75 (2001). Bhat RV et al. Regulation and localization of tyrosine216 phosphorylation of glycogen synthase kinase-3beta in cellular and animal models of neuronal degeneration. Proc Natl Acad Sci U S A 97:11074-9 (2000). General / background references:Brady MJ et al. The activation of glycogen synthase by insulin switches from kinase inhibition to phosphatase activation during adipogenesis in 3T3-L1 cells. J Biol Chem 273:14063-6 (1998). |
| Phosphorylated: | | Tyr216/Tyr279 |
| more information: | | company product webpage |