| CatalogCode: | NSB557 |
| ProductName: | SHP2 Antibody |
| Product Description: | Rabbit Polyclonal anti-SHP2 [Ser576] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human SHP2 that contains serine 576. The sequence is conserved in mouse, rat and chicken. |
| CrossReactivity: | Human SHP2. Mouse and rat SHP2 (100% homologous) have not been tested, but are expected to react. |
| Packaging: | 0.1 ml of epitope affinity purified rabbit antisera in PBS(without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1 mg/ml BSA (IgG, protease free) as a carrier. Contians 0.05% sodium azide (Caution: sodium azide is a poisonous and hazardous substance. Handle with care and dispose of properly.) |
| Uses: | This antibody is suitable for use in Western blotting. The recommended concentration for use in Western blotting is 1:1,000.*The optimal concentration should be determined for each specific application. |
| Control: | Hek293 treated with PMA. |
| Background: | SHP2 (SH2-domain containing tyrosine phosphatase 2, also known as SHPTP2) is a ~70 kDa tyrosine phosphatase that plays an important role in cell growth and transformation and in mediating integrin, Src and receptor tyrosine kinase (RTK) signaling. SHP2 undergoes tyrosine phosphorylation on several sites including tyrosines 542 and 580 upon binding to platelet-derived growth factor receptor beta (PDGFRb), in response to PDGF stimulation. Serines 576 and 591 are phosphorylated by protein kinase C isoforms alpha, beta 1, beta 2, and eta, and in response to phorbol ester. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-BPTP3 antibody, anti-CFC antibody, anti-Noonan syndrome 1 protein tyrosine phosphatase 2C antibody, anti-NS1 antibody, anti-Protein Tyrosine Phosphatase Non receptor Type 11 antibody, anti-PTP1D antibody, anti-PTP2C antibody, anti-PTPN11 antibody, anti-SAP2 antibody, anti-SH2 domain containing protein tyrosine phosphatase 2 antibody, anti-SHPTP2 antibody, anti-SHPTP3 antibody, anti-SIT protein precursor antibody |
| ProteinTarget: | SHP2 (phospho S576) |
| PackageSize: | 0.1 ml |
| GeneralRef: | Zhang, S.Q., et al. (2004) Shp2 regulates SRC family kinase activity and Ras/Erk activation by controlling Csk recruitment. Mol. Cell. 13(3):341-355. Araki, T., et al. (2003) Tyrosyl phosphorylation of Shp2 is required for normal ERK activation in response to some, but not all, growth factors. J. Biol. Chem. 278(43):41677-41684. Chen, Y., et al. (2003) Identification of Shp-2 as a Stat5A phosphatase. J. Biol. Chem. 278(19): 16520-16527. MacGillivray, M., et al. (2003) The protein tyrosine phosphatase SHP-2 regulates interleukin-1-induced ERK activation in fibroblasts. J. Biol. Chem. 278(29):27190-27198. Strack, V., et al. (2002) The Protein-tyrosine-phosphatase SHP2 is phosphorylated on serine residues 576 and 591 by protein kinase C isoforms alpha, beta 1, beta 2, and eta. Biochemistry 41(2):603-608. Vadlamudi, R.K., et al. (2002) Differential regulation of components of the focal adhesion complex by heregulin: role of phosphatase SHP-2. J. Cell. Physiol. 190(2):189-199. Seki, N., et al. (2002) Role of SRC homology 2-containing tyrosine phosphatase 2 on proliferation of rat smooth muscle cells. Arterioscler. Thromb. Vasc. Biol. 22(7):1081-1085. Lu, W., et al. (2001) Site-specific incorporation of a phosphotyrosine mimetic reveals a role for tyrosine phosphorylation of SHP-2 in cell signaling. Mol. Cell. 8(4):759-769. Bennett, A.M., et al. (1994) Protein-tyrosine-phosphatase SHPTP2 couples platelet-derived growth factor receptor beta to Ras. Proc. Nat'l. Acad. Sci. USA 91(15):7335-7339. |
| Phosphorylated: | Ser576 |
|