| CatalogCode: | | NSB516 |
| ProductName: | | MAPKAPK-2 Antibody |
| Product Description: | | Rabbit Polyclonal anti-MAPKAPK-2 [Thr334] |
| Clonality: | | Polyclonal |
| Immunogen: | | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MAPKAPK-2 that contains threonine 334. The sequence is conserved in mouse, hamster and rabbit. |
| CrossReactivity: | | Human and mouse MAPKAPK-2. Hamster and rabbit MAPKAPK-2 (100% homologous) have not been tested, but are expected to react. |
| Packaging: | | 0.1 ml of epitope-specific affinity purified rabbit antisera in PBS (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1 mg/ml BSA (IgG, protease free) as a carrier. Contains 0.05% sodium azide (Caution: sodium azide is a poisonous and hazardous substance. Handle with care and dispose of properly.) |
| Uses: | | The antibody has been used for Western blotting. No other applications have been tested.Recommended starting dilutions:Western Blot - 1:1000 *The optimal antibody concentration should be determined empirically for each specific application. |
| Control: | | HeLa cells treated with TNF-a or anisomycin; NIH-3T3 cells treated with anisomycin. |
| Background: | | Mitogen activated protein kinase-activated protein kinase-2 (MAPKAPK-2), expressed as alpha (~49 kDa) and beta (~60 kDa) isoforms, is phosphorylated by p38 MAPK and mediates multiple p38 MAPK-dependent cellular responses, including inflammation. MAPKAPK-2 is implicated in several disorders including brain ischemic injury and heart failure, and has shown to be important in regulating stress resistance and the production of tumor necrosis factor alpha (TNF-a). In unstimulated cells, MAPKAPK-2 is located in the nucleus where it contributes to CREB phosphorylation. Upon stimulation it translocates to the cytoplasm and phosphorylates and activates tuberin and HSP27. MAPKAPK-2 is phosphorylated in the kinase domain at threonine 222 (in the activation loop) and serine 272, and in the C-terminus at threonines 334 and 338. Threonine 334 is a major regulatory site, as once phosphorylated it induces conformational changes which result in the activation of the kinase. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Host_Name: | | Rabbit |
| ListPrice: | | 325 |
| AppSummary: | | WB |
| SpeciesSummary: | | Hu, Mu |
| ProteinTarget: | | MAPKAPK-2, phospho |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | Li, Y., et al. (2003) The p38 and MK2 kinase cascade phosphorylates tuberin, the tuberous sclerosis 2 gene product, and enhances its interaction with 14-3-3. J. Biol. Chem. 278(16):13663-13671. Song, C., et al. (2002) beta-Amyloid peptide induces formation of actin stress fibers through p38 mitogen-activated protein kinase. J. Neurochem. 83(4):828-836. Wang, X., et al. (2002) Mitogen-activated protein kinase-activated protein (MAPKAP) kinase 2 deficiency protects brain from ischemic injury in mice. J. Biol. Chem. 277(46):43968-43972.Nakano, A., et al. (2000) Ischemic preconditioning activates MAPKAPK2 in the isolated rabbit heart: evidence for involvement of p38 MAPK. Circ. Res. 86(2):144-151. Verma, A., et al. (2000) Cutting edge: activation of the p38 mitogen-activated protein kinase signaling pathway mediates cytokine-induced hemopoietic suppression in aplastic anemia. J. Immunol. 168(12):5984-5988. Chevalier, D. and B.G. Allen (2000) Two distinct forms of MAPKAP kinase-2 in adult cardiac ventricular myocytes. Biochemistry. 39(20):6145-6156. Larsen, J.K., et al. (1997) Phosphorylation of the 27-kDa heat shock protein via p38 MAP kinase and MAPKAP kinase in smooth muscle. Am. J. Physiol. 273(5 Pt 1):L930-L940. Engel, K., et al. (1995) MAPKAP kinase 2 is activated by heat shock and TNF-alpha: in vivo phosphorylation of small heat shock protein results from stimulation of the MAP kinase cascade. J. Cell. Biochem. 57(2):321-330. Nakano, A., et al. Ischemic preconditioning activates MAPKAPK2 in the isolated rabbit heart:evidence for involvement of p38 MAPK. Circ. Res. 86(2): 144-151, 2000. |
| Phosphorylated: | | Thr334 |
| more information: | | company product webpage |
|