| CatalogCode: | | NSB505 |
| ProductName: | | c-Raf Antibody |
| Product Description: | | Rabbit Polyclonal anti-c-Raf [Ser259] |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide (Human) derived from a region of human c-Raf that contains serine 259. The sequence is conserved in mouse and rat. |
| Specificity: | | A-Raf (90%) was also shown to react in HCT-8 cells, a human cancer cell line expressing high levels of A-Raf. |
| CrossReactivity: | | Cross-reacts with Human. Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | | 0.1 ml |
| Uses: | | WB: Use at a concentration of 1:1,000. Predicted molecular weight: 74 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Control: | | Immunoprecipitates of EGF-stimulated Hek293 cells transfected with c-Raf, and HCT-8 cells. |
| Background: | | The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Serine 259 is one of the three constitutive phosphorylation sites of c-Raf in resting cells together with serine 43 and serine 621. Serine 259 is phosphorylated by PKA and is the main mediator of PKA-induced inhibition of c-Raf kinase activity. Phosphorylation of serine 259 blocks the membrane localization of c-Raf. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | Preservative: 0.05% Sodium AzideConstituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.3 |
| ListPrice: | | 325 |
| AppSummary: | | WB |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-Craf 1 transforming gene antibody, anti-cRaf antibody, anti-Craf1 transforming gene antibody, anti-Murine sarcoma 3611 oncogene 1 antibody, anti-RAF antibody, anti-Raf proto oncogene serine/threonine protein kinase antibody, anti-vraf1 murine leukemia viral oncogene homolog 1 antibody |
| ProteinTarget: | | c-Raf phospho |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | <b>General / background references:</b>Dhillon AS <i>et al.</i> Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259. <i>Mol Cell Biol</i> <b>22</b>:3237-46 (2002). <PUBMED:11971957>Dumaz N <i>et al.</i> Cyclic AMP blocks cell growth through Raf-1-dependent and Raf-1-independent mechanisms. <i>Mol Cell Biol</i> <b>22</b>:3717-28 (2002). <PUBMED:11997508>Zhang BH & Guan KL Regulation of the Raf kinase by phosphorylation. <i>Exp Lung Res</i> <b>27</b>:269-95 (2001). <PUBMED:11293329>Morrison DK <i>et al.</i> Identification of the major phosphorylation sites of the Raf-1 kinase. <i>J Biol Chem</i> <b>268</b>:17309-16 (1993). <PUBMED:8349614> 5. Carey, K.D., et al. The requirement of specific membrane domains for Raf-1 phosphrylation and activations. J. Biol. Chem. 278(5):3185-3196, 2003. 6. Mason, C.S., et al. Serine and tyrosine phosphorylation cooperate in Raf-1, but not B-Raf activation. EMBO J. 18(8): 2137-2148, 1999. 7. Diaz, B., et al. Phosphorylation of Raf-1 serine 338-serine 339 is an essential regulatory event for Ras-dependent activation and biological signaling. Mol. Cell Biol. 17(8):4509-4516, 1997. 8. Marais, R., et al. Ras recruits Raf-1 to the plasma membrance for activation by tyrosine phosphorylation. EMBO J. 14(13):3136-3145, 1995. |
| Phosphorylated: | | Ser259 |
| more information: | | company product webpage |
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