| CatalogCode: | NSB504 |
| ProductName: | c-Raf Antibody |
| Product Description: | Rabbit Polyclonal anti-c-Raf [Ser621] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (Human) derived from a region of human c-Raf that contains serine 621. The sequence is conserved in mouse and rat. |
| Specificity: | A-Raf and B-Raf (89%) have not been tested, but may cross-react in cells expressing high levels of these proteins. |
| CrossReactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Predicted molecular weight: 74 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Control: | Immunoprecipitates or cell lysates from EGF-stimulated Hek293 cells transfected with c-Raf. |
| Background: | The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated phosphorylates MEK which in turn phosphorylates ERK. Together with serine 43 and serine 259, serine 621 is one of the three constitutive phosphorylation sites of c-Raf in resting cells. Phosphorylation of serine 621 has been shown to positively or negatively regulate c-Raf kinase activity, depending on the system. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Constituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.3 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Craf 1 transforming gene antibody; anti-cRaf antibody; anti-Craf1 transforming gene antibody; anti-Murine sarcoma 3611 oncogene 1 antibody; anti-RAF antibody; anti-Raf proto oncogene serine/threonine protein kinase antibody; anti-vraf1 murine leukemia viral oncogene homolog 1 antibody |
| ProteinTarget: | c-Raf phospho |
| PackageSize: | 0.1 ml |
| GeneralRef: | This product has been used in:Gu M et al. Nitric oxide increases p21(Waf1/Cip1) expression by a cGMP-dependent pathway that includes activation of extracellular signal-regulated kinase and p70(S6k). J Biol Chem 275:11389-96 (2000). Thorson JA et al. 14-3-3 proteins are required for maintenance of Raf-1 phosphorylation and kinase activity. Mol Cell Biol 18:5229-38 (1998). General / background references:Dhillon AS et al. Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259. Mol Cell Biol 22:3237-46 (2002). Dumaz N et al. Cyclic AMP blocks cell growth through Raf-1-dependent and Raf-1-independent mechanisms. Mol Cell Biol 22:3717-28 (2002). Zhang BH & Guan KL Regulation of the Raf kinase by phosphorylation. Exp Lung Res 27:269-95 (2001). Mischak H et al. Negative regulation of Raf-1 by phosphorylation of serine 621. Mol Cell Biol 16:5409-18 (1996). |
| Phosphorylated: | Ser621 |
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